Detailed information NTG1166580

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB5Y62_RS02685
• Genome accession: NZ_OZ078860
• Coordinates: 540334..541203 (-)
• Length: 289 a.a.
• NCBI ID: WP_017087402.1
• Uniprot ID: A0A2N7FBH3
• Organism: Vibrio splendidus isolate A06
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 535334..546203

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB5Y62_RS02650	rpsP	536316..536564 (+)	249	WP_004735508.1	30S ribosomal protein S16	-
AB5Y62_RS02655	rimM	536589..537143 (+)	555	WP_004735510.1	ribosome maturation factor RimM	-
AB5Y62_RS02660	trmD	537170..537910 (+)	741	WP_004735512.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
AB5Y62_RS02665	rplS	537970..538323 (+)	354	WP_004735514.1	50S ribosomal protein L19	-
AB5Y62_RS02670	yacG	538671..538868 (-)	198	WP_004735515.1	DNA gyrase inhibitor YacG	-
AB5Y62_RS02675	zapD	538950..539690 (-)	741	WP_368084106.1	cell division protein ZapD	-
AB5Y62_RS02680	coaE	539717..540331 (-)	615	WP_368084107.1	dephospho-CoA kinase	-
AB5Y62_RS02685	pilD	540334..541203 (-)	870	WP_017087402.1	A24 family peptidase	Machinery gene
AB5Y62_RS02690	pilC	541271..542500 (-)	1230	WP_256799836.1	type II secretion system F family protein	Machinery gene
AB5Y62_RS02695	pilB	542515..544200 (-)	1686	WP_368084938.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AB5Y62_RS02700	pilA	544205..544630 (-)	426	WP_368084108.1	pilin	Machinery gene
AB5Y62_RS02705	nadC	544886..545776 (-)	891	WP_102448387.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32551.81 Da
• Isoelectric Point: 7.6347

>NTDB_id=1166580 AB5Y62_RS02685 WP_017087402.1 540334..541203(-) (pilD) [Vibrio splendidus isolate A06]
MEVFHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPAPEGKFNLSIPRSTCPKCKTQLRII
DNIPVLSWLLLKGKCHSCANPISARYPLVELLTAILCTVVASHFGFSYYAIALIFFTFALITATFIDLDTMLLPDQITLP
LVWSGIALALFNISPVSLQDSVIGAMAGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
VFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLMWGNDIMGWYFTSVLGI

Nucleotide

• Length: 870 bp

>NTDB_id=1166580 AB5Y62_RS02685 WP_017087402.1 540334..541203(-) (pilD) [Vibrio splendidus isolate A06]
ATGGAAGTATTTCACTACTATCCTTGGCTATTCCCCGTATTAGCTTTCATTTTTAGCCTTCTTATCGGCAGCTTCCTCAA
CGTAGTCATCCACCGTTTACCGATAATGATGGAACGAGAGTGGCAACAAGAGTGCTCGGAGTATTTCTCTCAATATAAAA
TTCCAGCACCAGAGGGAAAGTTCAATCTCAGTATCCCTCGTTCTACCTGCCCGAAATGTAAAACCCAATTAAGAATCATC
GACAATATTCCAGTATTAAGTTGGTTGTTATTAAAGGGCAAGTGTCATAGCTGTGCTAACCCAATCAGCGCTCGTTACCC
ATTGGTCGAACTGCTTACGGCGATACTTTGTACCGTAGTCGCGAGCCACTTTGGTTTCAGTTACTACGCCATTGCTTTGA
TTTTTTTCACGTTTGCATTGATTACCGCAACCTTTATCGATCTCGATACTATGCTGTTGCCTGATCAAATTACCTTGCCT
TTAGTTTGGTCTGGTATCGCGTTAGCTCTGTTTAATATCAGCCCTGTATCGCTTCAAGATTCAGTGATTGGCGCAATGGC
AGGCTACCTAGCACTTTGGTCGGTTTATTGGCTGTTCAAGCTGTTAACAGGCAAAGAAGGTATGGGTTACGGAGACTTCA
AACTTCTGGCTGCGCTTGGCGCCTGGCTTGGCTGGCAGCACCTACCAATGATCATTCTTTTGTCGTCGCTGGTCGGTTTA
GTTTTCGGCTTGATTCAACTTCGCTTGAAACAACAAGGCATAGATAAAGCCTTTCCATTTGGACCTTACCTTGCAATTGC
GGGTTGGGTGAGTTTGATGTGGGGTAACGACATCATGGGTTGGTATTTCACTTCTGTACTAGGAATTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A2N7FBH3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	75.087	100	0.751
pilD	Vibrio cholerae strain A1552	69.444	99.654	0.692
pilD	Acinetobacter nosocomialis M2	51.311	92.388	0.474
pilD	Acinetobacter baumannii D1279779	50.936	92.388	0.471
pilD	Neisseria gonorrhoeae MS11	46.739	95.502	0.446