Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RG43_RS26805 Genome accession   NZ_CP010157
Coordinates   5082897..5084390 (+) Length   497 a.a.
NCBI ID   WP_000249319.1    Uniprot ID   -
Organism   Escherichia coli strain D10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5077897..5089390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG43_RS26785 (RG43_25225) pilD 5078545..5079354 (+) 810 WP_001318036.1 prepilin peptidase PppA Machinery gene
  RG43_RS26790 (RG43_25230) gspS2 5079420..5079830 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  RG43_RS26795 (RG43_25235) gspC 5079848..5080807 (+) 960 WP_001696202.1 type II secretion system protein GspC -
  RG43_RS26800 (RG43_25240) gspD 5080837..5082897 (+) 2061 WP_001318034.1 type II secretion system secretin GspD -
  RG43_RS26805 (RG43_25245) pilB/pilB1 5082897..5084390 (+) 1494 WP_000249319.1 type II secretion system ATPase GspE Machinery gene
  RG43_RS26810 (RG43_25250) gspF 5084390..5085613 (+) 1224 WP_001173415.1 type II secretion system inner membrane protein GspF -
  RG43_RS26815 (RG43_25255) gspG 5085630..5086085 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  RG43_RS26820 (RG43_25260) gspH 5086089..5086652 (+) 564 WP_001115149.1 type II secretion system minor pseudopilin GspH -
  RG43_RS26825 (RG43_25265) gspI 5086649..5087020 (+) 372 WP_000820091.1 type II secretion system minor pseudopilin GspI -
  RG43_RS26830 (RG43_25270) gspJ 5087017..5087622 (+) 606 WP_001318033.1 type II secretion system minor pseudopilin GspJ -
  RG43_RS26835 (RG43_25275) gspK 5087619..5088596 (+) 978 WP_073506912.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55178.36 Da        Isoelectric Point: 5.9081

>NTDB_id=116555 RG43_RS26805 WP_000249319.1 5082897..5084390(+) (pilB/pilB1) [Escherichia coli strain D10]
MVPVAQETTANTVRLPYSFSRRFGLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSVPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=116555 RG43_RS26805 WP_000249319.1 5082897..5084390(+) (pilB/pilB1) [Escherichia coli strain D10]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTGGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTACAGGAGCTACAGCGGGGGCTGA
ATGCGCCCTTTACACTGCGGCAAATCGACGAGGCTGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTTGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGTACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGCACCGCTATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45.25

80.483

0.364

Multiple sequence alignment