Detailed information NTG0116497

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AI22_RS01895
• Genome accession: NZ_CP007147
• Coordinates: 392414..393562 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa YL84
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 387414..398562

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AI22_RS01875 (AI22_01870)	-	388151..389335 (-)	1185	WP_025324561.1	cystathionine gamma-synthase	-
AI22_RS01880 (AI22_01875)	-	389332..390705 (-)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
AI22_RS01885 (AI22_01880)	-	390948..391352 (-)	405	WP_003084560.1	NINE protein	-
AI22_RS01890 (AI22_01885)	czcD	391507..392406 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
AI22_RS01895 (AI22_01890)	pilU	392414..393562 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
AI22_RS01900 (AI22_01895)	pilT	393740..394774 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
AI22_RS01905 (AI22_01900)	-	394989..395681 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
AI22_RS01910 (AI22_01905)	proC	395693..396514 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
AI22_RS01915 (AI22_01910)	-	396525..397118 (+)	594	WP_003084544.1	YggT family protein	-
AI22_RS01920 (AI22_01915)	-	397115..397411 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=116497 AI22_RS01895 WP_003100959.1 392414..393562(-) (pilU) [Pseudomonas aeruginosa YL84]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=116497 AI22_RS01895 WP_003100959.1 392414..393562(-) (pilU) [Pseudomonas aeruginosa YL84]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382