Detailed information NTG0116485

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: YC6258_RS20780
• Genome accession: NZ_CP007142
• Coordinates: 4973586..4974758 (-)
• Length: 390 a.a.
• NCBI ID: WP_044618621.1
• Uniprot ID: A0A0C5VPW7
• Organism: Gynuella sunshinyii YC6258
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4968586..4979758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
YC6258_RS20765 (YC6258_04627)	-	4970295..4971791 (+)	1497	WP_169749004.1	PfaD family polyunsaturated fatty acid/polyketide biosynthesis protein	-
YC6258_RS20770 (YC6258_04628)	-	4971801..4973195 (+)	1395	WP_044618619.1	alpha-amylase family glycosyl hydrolase	-
YC6258_RS20775 (YC6258_04629)	-	4973260..4973526 (-)	267	WP_044618620.1	DksA/TraR family C4-type zinc finger protein	-
YC6258_RS20780 (YC6258_04630)	pilU	4973586..4974758 (-)	1173	WP_044618621.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
YC6258_RS27600 (YC6258_04632)	-	4974929..4976596 (-)	1668	WP_052830451.1	PAS domain S-box protein	-
YC6258_RS20790 (YC6258_04634)	-	4976998..4977405 (-)	408	WP_044618622.1	GFA family protein	-
YC6258_RS20795 (YC6258_04635)	-	4977961..4979181 (-)	1221	WP_044618623.1	SAVED domain-containing protein	-
YC6258_RS27605	-	4979178..4979696 (-)	519	WP_052830452.1	polymorphic toxin-type HINT domain-containing protein	-

Sequence

Protein

• Length: 390 a.a.
• Molecular weight: 43077.42 Da
• Isoelectric Point: 6.3232

>NTDB_id=116485 YC6258_RS20780 WP_044618621.1 4973586..4974758(-) (pilU) [Gynuella sunshinyii YC6258]
MATPIDQYLKLLATKDGSDLYLSTGAPPCAKFQGSLKPVSQTPLKPGEIENIANQIMDEEQRREFAQELEMNLAISISGV
GRFRINIFKQRNEVSIVARNINTEIPQFDHLGLPPVLKDVVMTKRGLVLFVGGTGSGKSTSLAALIDHRNSNSGGHIITI
EDPVEFVHKHKKSIINQREVGVDTRSFRNALKNTLRQAPDVILIGEIRDRETMEHALEFAETGHLAISTLHANNANQALE
RIINMFPEERRPQLLLGLAQNIRAIVSQRLIPTVDGKRCAAVEVLLGTKTIQELILKARLEDIKEIMEKSENLGMQTFDA
AIFKLYQAGKITLTEALANADSPNNLRLKIKLASEQNKSPEIGEKNAEPISSGFGELKLEVLGDTDEEGL

Nucleotide

• Length: 1173 bp

>NTDB_id=116485 YC6258_RS20780 WP_044618621.1 4973586..4974758(-) (pilU) [Gynuella sunshinyii YC6258]
ATGGCCACACCAATTGACCAGTATCTGAAGCTGTTGGCGACAAAAGATGGGTCTGATCTGTATCTCAGTACCGGCGCACC
ACCCTGTGCGAAGTTTCAGGGTTCCCTGAAGCCGGTCTCACAAACACCCCTGAAACCGGGTGAAATTGAAAATATTGCCA
ATCAGATCATGGATGAAGAGCAGCGCCGGGAATTCGCCCAGGAACTGGAAATGAATCTGGCCATCTCCATCTCCGGAGTT
GGTCGTTTCCGTATTAACATCTTTAAACAACGTAACGAAGTCTCCATCGTCGCCCGAAATATCAATACCGAAATTCCCCA
ATTCGATCACCTGGGATTGCCGCCAGTGCTCAAGGATGTGGTAATGACCAAGCGCGGGCTGGTGTTGTTTGTCGGTGGTA
CCGGGTCTGGTAAATCCACCTCTCTGGCGGCATTGATAGATCATCGTAACTCCAACTCGGGTGGCCATATCATCACTATC
GAGGACCCGGTTGAGTTTGTCCATAAACACAAAAAAAGCATCATCAATCAGCGTGAAGTGGGTGTGGATACCCGCAGTTT
TCGCAATGCCTTGAAAAACACTTTGCGACAGGCACCGGACGTGATTCTGATTGGTGAGATTCGTGATCGGGAAACCATGG
AACACGCGCTCGAGTTTGCCGAAACCGGTCACCTGGCCATTTCCACCCTGCATGCCAATAACGCGAACCAGGCGCTGGAG
CGCATCATTAATATGTTTCCGGAAGAACGTCGGCCACAGTTGCTGTTGGGGTTGGCGCAGAATATTCGTGCGATTGTGTC
ACAGCGGTTGATTCCCACAGTGGATGGAAAACGCTGCGCAGCCGTCGAAGTGTTGCTGGGCACTAAAACCATTCAGGAAC
TGATCCTGAAAGCCCGGTTGGAAGACATTAAAGAAATCATGGAAAAATCTGAAAACCTTGGCATGCAGACATTCGATGCG
GCCATTTTCAAGCTGTATCAGGCTGGTAAAATCACTCTGACCGAAGCATTGGCCAACGCCGATTCACCCAATAACCTGCG
TTTGAAAATCAAACTCGCGAGTGAACAAAACAAGTCTCCGGAGATTGGTGAAAAAAATGCTGAACCGATCAGTTCCGGTT
TTGGCGAACTGAAATTGGAAGTGTTGGGTGACACCGATGAAGAGGGGCTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (82-272)

3D structure

• AlphaFold DB: A0A0C5VPW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.932	90.769	0.508
pilU	Acinetobacter baylyi ADP1	53.039	92.821	0.492
pilU	Vibrio cholerae strain A1552	48.851	89.231	0.436
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.426	96.41	0.39