Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   YC6258_RS04570 Genome accession   NZ_CP007142
Coordinates   1031282..1032340 (+) Length   352 a.a.
NCBI ID   WP_211264624.1    Uniprot ID   A0A0C5VFX0
Organism   Gynuella sunshinyii YC6258     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1006883..1036358 1031282..1032340 within 0


Gene organization within MGE regions


Location: 1006883..1036358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  YC6258_RS04465 (YC6258_01024) - 1006883..1007482 (-) 600 WP_044615978.1 6-carboxytetrahydropterin synthase -
  YC6258_RS04470 (YC6258_01025) - 1007502..1008374 (-) 873 WP_044615979.1 hypothetical protein -
  YC6258_RS04475 (YC6258_01026) folE 1008401..1009018 (-) 618 WP_044615980.1 GTP cyclohydrolase I FolE -
  YC6258_RS04480 (YC6258_01027) - 1009061..1010215 (-) 1155 WP_044615981.1 IMP dehydrogenase -
  YC6258_RS04485 (YC6258_01028) - 1010322..1011002 (-) 681 WP_044615982.1 HAD family hydrolase -
  YC6258_RS27005 (YC6258_01029) - 1011030..1011551 (-) 522 WP_052830053.1 phosphoribosyltransferase domain-containing protein -
  YC6258_RS04495 (YC6258_01030) - 1011562..1012554 (-) 993 WP_044615983.1 adenylosuccinate synthetase -
  YC6258_RS04500 (YC6258_01031) purB 1012692..1014044 (-) 1353 WP_044615984.1 adenylosuccinate lyase -
  YC6258_RS04505 (YC6258_01032) queC 1014099..1014782 (-) 684 WP_044615985.1 7-cyano-7-deazaguanine synthase QueC -
  YC6258_RS04510 (YC6258_01034) hslU 1015850..1017178 (-) 1329 WP_044615986.1 ATP-dependent protease ATPase subunit HslU -
  YC6258_RS04515 (YC6258_01035) hslV 1017210..1017728 (-) 519 WP_044615987.1 ATP-dependent protease subunit HslV -
  YC6258_RS04520 (YC6258_01036) - 1017792..1018403 (-) 612 WP_169748931.1 SPOR domain-containing protein -
  YC6258_RS04525 (YC6258_01037) - 1018529..1020883 (-) 2355 WP_144407560.1 primosomal protein N' -
  YC6258_RS04530 rpmE 1020953..1021171 (+) 219 WP_044615990.1 50S ribosomal protein L31 -
  YC6258_RS04535 (YC6258_01039) mdh 1021367..1022308 (+) 942 WP_044615991.1 malate dehydrogenase -
  YC6258_RS04540 (YC6258_01040) - 1022354..1022968 (-) 615 WP_044615992.1 TetR/AcrR family transcriptional regulator -
  YC6258_RS04545 (YC6258_01041) - 1023072..1024163 (+) 1092 WP_044615993.1 efflux RND transporter periplasmic adaptor subunit -
  YC6258_RS04550 (YC6258_01042) - 1024163..1027225 (+) 3063 WP_082070559.1 efflux RND transporter permease subunit -
  YC6258_RS27010 (YC6258_01043) - 1027251..1028135 (-) 885 WP_052830054.1 LysR family transcriptional regulator -
  YC6258_RS04560 (YC6258_01044) trxA 1028238..1028579 (+) 342 WP_044615994.1 thioredoxin -
  YC6258_RS04565 (YC6258_01045) - 1028576..1031107 (-) 2532 WP_052830055.1 penicillin-binding protein 1A -
  YC6258_RS04570 (YC6258_01046) pilM 1031282..1032340 (+) 1059 WP_211264624.1 pilus assembly protein PilM Machinery gene
  YC6258_RS04575 (YC6258_01047) - 1032343..1032918 (+) 576 WP_044615996.1 PilN domain-containing protein -
  YC6258_RS04580 (YC6258_01048) pilO 1032918..1033562 (+) 645 WP_044615997.1 type 4a pilus biogenesis protein PilO -
  YC6258_RS04585 (YC6258_01049) - 1033559..1034086 (+) 528 WP_044615998.1 pilus assembly protein PilP -
  YC6258_RS04590 (YC6258_01050) - 1034124..1035644 (+) 1521 WP_052830056.1 AMIN domain-containing protein -
  YC6258_RS30845 pilQ 1035645..1036358 (+) 714 WP_052830057.1 hypothetical protein Machinery gene

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38293.53 Da        Isoelectric Point: 4.2755

>NTDB_id=116469 YC6258_RS04570 WP_211264624.1 1031282..1032340(+) (pilM) [Gynuella sunshinyii YC6258]
MSLFKKKDNIVLGLDISSTSVKLLELSRNNDGYRVENYSVEPLPENAVVEKNINDVEGVGQAIAKLVAKTKTRRKKAAVA
VAGSAVITKTIEMNAGLTDDEMETQINVEADQYIPYPLDEVAIDFEVIGSSPNTDSMVEVLLAACRRENVEFREDSLEIG
GLESAIVDVEAFALERSFQLLVPQFESVDEDMTVAVVDVGHSMTTLSVLNNGKTIYTREQLFGGKQLTEEIMRRYGLTVQ
EAGLAKKQGGLSDDYATEVLEPFKEAVVQQVTRSLQFFFAASQYNDVDYIVLAGGTASIPGLAQLIQDKIGTATIVANPF
ANMTLANRVNAANLTNDAPALMICCGLAMRSF

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=116469 YC6258_RS04570 WP_211264624.1 1031282..1032340(+) (pilM) [Gynuella sunshinyii YC6258]
GTGAGTCTCTTCAAGAAAAAGGACAACATCGTTTTGGGTCTGGACATCAGTTCCACTTCTGTGAAGTTGTTGGAGTTGAG
TCGTAATAACGATGGTTATCGGGTAGAAAACTATTCTGTCGAGCCATTACCTGAAAACGCTGTTGTTGAGAAGAACATTA
ATGATGTTGAAGGTGTCGGCCAGGCGATCGCCAAACTTGTGGCTAAAACGAAAACCCGTCGCAAGAAGGCTGCCGTTGCA
GTAGCTGGTTCCGCTGTCATCACTAAAACCATCGAAATGAATGCTGGTCTTACGGATGATGAGATGGAAACACAGATTAA
TGTTGAGGCAGATCAATATATTCCATACCCACTTGACGAAGTTGCCATAGACTTTGAAGTAATCGGATCATCACCGAATA
CAGATAGCATGGTAGAGGTATTGTTGGCAGCCTGCCGACGGGAAAATGTCGAGTTTCGGGAAGATTCTCTGGAAATCGGT
GGACTGGAAAGCGCTATTGTAGATGTTGAGGCATTTGCTTTGGAGCGCTCATTTCAGCTGCTGGTGCCGCAGTTTGAATC
CGTCGATGAGGACATGACGGTCGCCGTTGTAGATGTTGGTCACTCAATGACCACGCTAAGCGTATTGAATAATGGCAAGA
CGATATATACCCGCGAGCAACTCTTCGGTGGTAAACAATTGACTGAGGAAATCATGCGCCGCTATGGACTGACTGTTCAG
GAAGCTGGACTGGCCAAAAAACAGGGTGGTTTATCTGATGACTATGCAACTGAGGTTCTTGAGCCTTTTAAAGAGGCAGT
GGTTCAACAGGTGACCCGGTCTCTGCAGTTTTTCTTTGCTGCCAGTCAATACAATGACGTGGACTATATCGTATTGGCCG
GAGGTACCGCCTCTATTCCCGGATTGGCTCAGTTAATTCAGGACAAAATCGGTACTGCAACGATTGTTGCCAATCCATTT
GCTAACATGACGCTGGCGAACAGAGTAAATGCCGCTAATCTGACCAATGATGCTCCAGCATTAATGATTTGCTGTGGTTT
AGCGATGAGGAGCTTCTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C5VFX0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.557

100

0.526
  comM Acinetobacter nosocomialis M2

52.557

100

0.526
  comM Acinetobacter baylyi ADP1

50.142

100

0.503
  pilM Legionella pneumophila strain ERS1305867

45.299

99.716

0.452

Multiple sequence alignment