Detailed information NTG1163299

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ABNT83_RS09370
• Genome accession: NZ_OZ026884
• Coordinates: 1997360..1998424 (+)
• Length: 354 a.a.
• NCBI ID: WP_348757307.1
• Uniprot ID: -
• Organism: Candidatus Methylocalor cossyra isolate MeCH1-AG
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1992360..2003424

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABNT83_RS09350 (MECH1_V1_1952)	-	1992831..1993610 (-)	780	WP_348757303.1	exodeoxyribonuclease III	-
ABNT83_RS09355 (MECH1_V1_1953)	rpmE	1993793..1993996 (+)	204	WP_348757304.1	50S ribosomal protein L31	-
ABNT83_RS09360 (MECH1_V1_1954)	-	1994048..1994665 (-)	618	WP_348757305.1	hypothetical protein	-
ABNT83_RS09365 (MECH1_V1_1955)	-	1994680..1997166 (-)	2487	WP_348757306.1	penicillin-binding protein 1A	-
ABNT83_RS09370 (MECH1_V1_1956)	pilM	1997360..1998424 (+)	1065	WP_348757307.1	pilus assembly protein PilM	Machinery gene
ABNT83_RS09375 (MECH1_V1_1957)	-	1998421..1999005 (+)	585	WP_348757308.1	PilN domain-containing protein	-
ABNT83_RS09380 (MECH1_V1_1958)	-	1999002..1999655 (+)	654	WP_348757309.1	type 4a pilus biogenesis protein PilO	-
ABNT83_RS09385 (MECH1_V1_1959)	-	1999777..2000250 (+)	474	WP_348757310.1	pilus assembly protein PilP	-
ABNT83_RS09390 (MECH1_V1_1960)	-	2000247..2002496 (+)	2250	WP_348757311.1	type IV pilus secretin PilQ	-
ABNT83_RS09395 (MECH1_V1_1961)	aroK	2002597..2003139 (+)	543	WP_348757312.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38380.76 Da
• Isoelectric Point: 4.4737

>NTDB_id=1163299 ABNT83_RS09370 WP_348757307.1 1997360..1998424(+) (pilM) [Candidatus Methylocalor cossyra isolate MeCH1-AG]
MFFFNRKKPELLGIDISSAAVKLLELSRLGSHYRVEGYAIVPLPPDAVVDKNVAKAEAIANALRVAIKQSGTRLKQAAVA
VPSSMVIAKVITMPASLGEDEMEAQIELEADQYIPYALDEVSLDFVVLGPTEQNPDTVDVLLVASRKENVEDRVAALELA
GLKAEIVDVESYALEHACAFILGQTAQGIEDSVTAIADVGASVTTLNVVHKHRVIYTREQNFGGKQLTEEIQRRYGLSYE
EAGLAKKYGGLPDNYLTEVLEPFKNALAQQISRSLQFFLSSTAQREVDRVILTGGCGSIPELDRYIEESLDIPTSVANPF
VNVALSPRVNAEALTSDAPALMTACGLALRSFDQ

Nucleotide

• Length: 1065 bp

>NTDB_id=1163299 ABNT83_RS09370 WP_348757307.1 1997360..1998424(+) (pilM) [Candidatus Methylocalor cossyra isolate MeCH1-AG]
ATGTTTTTTTTCAACCGCAAGAAGCCGGAACTTTTGGGCATCGATATCAGCTCGGCGGCGGTCAAGCTCCTTGAGCTCAG
CCGGCTAGGTTCGCACTACCGGGTCGAGGGTTACGCCATAGTGCCATTGCCCCCGGACGCCGTAGTCGACAAGAACGTTG
CGAAAGCCGAGGCCATTGCCAACGCCCTTAGGGTTGCCATCAAGCAGTCCGGTACCCGCCTGAAACAGGCGGCGGTCGCC
GTGCCCAGTTCCATGGTGATCGCCAAGGTCATCACCATGCCGGCCTCCCTCGGCGAGGACGAAATGGAAGCGCAAATCGA
GCTCGAAGCGGACCAGTACATCCCCTACGCCCTGGACGAGGTGAGCCTGGACTTCGTGGTGCTAGGACCCACGGAACAGA
ACCCCGACACGGTGGATGTATTGCTGGTGGCTTCGCGCAAAGAAAACGTGGAGGACCGGGTGGCGGCATTGGAACTGGCC
GGGTTGAAAGCCGAGATTGTGGACGTCGAATCCTATGCCCTGGAGCATGCTTGCGCCTTCATTCTTGGCCAGACGGCGCA
GGGAATCGAGGATTCGGTGACCGCCATCGCGGACGTCGGGGCAAGCGTGACGACCCTCAATGTCGTCCACAAACATCGGG
TCATTTATACCCGGGAGCAGAACTTCGGCGGTAAACAGCTTACCGAGGAAATCCAACGCCGCTACGGACTTTCCTATGAG
GAAGCGGGATTGGCAAAAAAATACGGCGGCTTGCCGGACAATTACCTCACCGAGGTCCTCGAGCCCTTCAAAAACGCCTT
GGCCCAGCAAATCAGCCGCTCCCTCCAGTTCTTTCTTTCCTCCACTGCCCAGCGCGAAGTCGATCGGGTGATCCTAACGG
GGGGCTGTGGGTCGATTCCAGAGCTCGATCGGTACATCGAAGAAAGCCTCGATATACCGACCTCCGTCGCCAACCCGTTC
GTCAACGTGGCGCTTTCTCCGCGCGTGAACGCAGAGGCTTTAACGAGCGACGCCCCTGCGCTGATGACGGCGTGCGGCCT
GGCGCTGAGGAGTTTCGACCAATGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.003	98.588	0.503
comM	Acinetobacter nosocomialis M2	51.003	98.588	0.503
comM	Acinetobacter baylyi ADP1	48.864	99.435	0.486
pilM	Legionella pneumophila strain ERS1305867	43.228	98.023	0.424