Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   PMCN07_RS01165 Genome accession   NZ_CP007040
Coordinates   235594..236016 (-) Length   140 a.a.
NCBI ID   WP_050951544.1    Uniprot ID   A0A5C6GZH4
Organism   Pasteurella multocida subsp. multocida str. HN07     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 188746..243668 235594..236016 within 0


Gene organization within MGE regions


Location: 188746..243668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMCN07_RS00875 (PMCN07_0167) - 189005..189847 (-) 843 WP_011271867.1 tyrosine-type recombinase/integrase -
  PMCN07_RS00880 (PMCN07_0168) mobH 189908..191809 (-) 1902 WP_076090444.1 MobH family relaxase -
  PMCN07_RS00890 (PMCN07_0169) - 192108..192716 (+) 609 WP_076090446.1 hypothetical protein -
  PMCN07_RS00895 - 192811..193128 (-) 318 WP_231116306.1 hypothetical protein -
  PMCN07_RS00900 (PMCN07_0170) - 193347..193538 (+) 192 WP_005653495.1 hypothetical protein -
  PMCN07_RS00905 (PMCN07_0171) - 193553..194143 (+) 591 WP_020910025.1 recombinase family protein -
  PMCN07_RS00910 (PMCN07_0172) - 194745..195506 (-) 762 WP_020910027.1 N-6 DNA methylase -
  PMCN07_RS00915 (PMCN07_0173) - 195610..196563 (-) 954 WP_020910028.1 ArdC family protein -
  PMCN07_RS11040 (PMCN07_0174) - 196694..196852 (-) 159 WP_005667862.1 hypothetical protein -
  PMCN07_RS00920 (PMCN07_0175) - 196856..197104 (-) 249 WP_005687602.1 hypothetical protein -
  PMCN07_RS00925 (PMCN07_0176) - 197107..197340 (-) 234 WP_005653503.1 hypothetical protein -
  PMCN07_RS00930 (PMCN07_0177) - 197425..197781 (-) 357 WP_044509376.1 hypothetical protein -
  PMCN07_RS00935 (PMCN07_0178) - 198162..198599 (+) 438 WP_020910030.1 TIGR03757 family integrating conjugative element protein -
  PMCN07_RS00940 (PMCN07_0179) - 198596..199537 (+) 942 WP_005687598.1 TIGR03756 family integrating conjugative element protein -
  PMCN07_RS00945 (PMCN07_0180) - 199552..201552 (+) 2001 WP_005687597.1 integrating conjugative element protein -
  PMCN07_RS00950 (PMCN07_0181) - 201568..201975 (+) 408 WP_005667853.1 hypothetical protein -
  PMCN07_RS00955 (PMCN07_0182) - 202020..202199 (-) 180 WP_047922037.1 hypothetical protein -
  PMCN07_RS00960 (PMCN07_0183) - 202396..202752 (+) 357 WP_047922052.1 hypothetical protein -
  PMCN07_RS00965 (PMCN07_0184) - 202779..204275 (-) 1497 WP_040975703.1 conjugal transfer protein TraG N-terminal domain-containing protein -
  PMCN07_RS00970 (PMCN07_0185) - 204284..204607 (-) 324 WP_005667837.1 hypothetical protein -
  PMCN07_RS00975 (PMCN07_0186) - 204617..205060 (-) 444 WP_005687593.1 hypothetical protein -
  PMCN07_RS00980 (PMCN07_0187) - 205032..207902 (-) 2871 WP_058230494.1 conjugative transfer ATPase -
  PMCN07_RS00985 (PMCN07_0188) - 207918..208325 (-) 408 WP_005653518.1 TIGR03751 family conjugal transfer lipoprotein -
  PMCN07_RS00990 (PMCN07_0189) - 208335..209735 (-) 1401 WP_005687589.1 TIGR03752 family integrating conjugative element protein -
  PMCN07_RS00995 (PMCN07_0190) - 209746..210615 (-) 870 WP_047922050.1 TIGR03749 family integrating conjugative element protein -
  PMCN07_RS01000 (PMCN07_0191) - 210615..211259 (-) 645 WP_047922049.1 PFL_4703 family integrating conjugative element protein -
  PMCN07_RS01005 (PMCN07_0192) - 211271..211642 (-) 372 WP_011271844.1 TIGR03750 family conjugal transfer protein -
  PMCN07_RS01010 (PMCN07_0193) - 211662..212063 (-) 402 WP_005653541.1 DUF2976 domain-containing protein -
  PMCN07_RS01015 (PMCN07_0194) - 212083..212328 (-) 246 WP_005750915.1 DUF3262 family protein -
  PMCN07_RS01020 (PMCN07_0195) - 212332..212652 (-) 321 WP_053007201.1 RAQPRD family integrative conjugative element protein -
  PMCN07_RS01025 (PMCN07_0196) - 212813..213499 (-) 687 WP_012564939.1 TIGR03747 family integrating conjugative element membrane protein -
  PMCN07_RS01030 (PMCN07_0197) - 213499..213834 (-) 336 WP_005687581.1 hypothetical protein -
  PMCN07_RS01035 (PMCN07_0198) traD 213818..216067 (-) 2250 WP_076090459.1 type IV conjugative transfer system coupling protein TraD -
  PMCN07_RS01040 (PMCN07_0199) - 216064..216570 (-) 507 WP_011271841.1 integrating conjugative element protein -
  PMCN07_RS01045 (PMCN07_0200) - 216586..217344 (-) 759 WP_011271840.1 transglycosylase SLT domain-containing protein -
  PMCN07_RS01050 (PMCN07_0201) - 217323..218063 (-) 741 WP_012564936.1 TIGR03759 family integrating conjugative element protein -
  PMCN07_RS01055 (PMCN07_0202) - 218067..218696 (-) 630 WP_012564935.1 lipoprotein -
  PMCN07_RS01060 (PMCN07_0203) - 218795..219526 (-) 732 WP_047922047.1 TraX family protein -
  PMCN07_RS01065 (PMCN07_0204) - 219617..219820 (-) 204 WP_047922046.1 hypothetical protein -
  PMCN07_RS01075 - 220099..221227 (+) 1129 Protein_205 IS4 family transposase -
  PMCN07_RS01080 (PMCN07_0207) - 221237..221362 (-) 126 WP_001303004.1 LysR family transcriptional regulator -
  PMCN07_RS01085 (PMCN07_0208) gltS 221593..222798 (-) 1206 WP_000599533.1 sodium/glutamate symporter -
  PMCN07_RS01090 - 223242..223562 (+) 321 WP_000562370.1 antibiotic biosynthesis monooxygenase family protein -
  PMCN07_RS01095 (PMCN07_0211) - 223555..223941 (+) 387 WP_000460651.1 hypothetical protein -
  PMCN07_RS01100 (PMCN07_0212) - 223949..224635 (+) 687 WP_001284954.1 ArsR/SmtB family transcription factor -
  PMCN07_RS01105 tetR(B) 224613..225235 (-) 623 Protein_211 tetracycline resistance transcriptional repressor TetR(B) -
  PMCN07_RS01110 (PMCN07_0215) tet(B) 225317..226522 (+) 1206 WP_001089068.1 tetracycline efflux MFS transporter Tet(B) -
  PMCN07_RS01115 (PMCN07_0216) tetC 226635..227228 (-) 594 WP_000428546.1 tetracyline resistance-associated transcriptional repressor TetC -
  PMCN07_RS01120 (PMCN07_0217) - 227316..227732 (+) 417 WP_000275180.1 helix-turn-helix domain-containing protein -
  PMCN07_RS01125 (PMCN07_0218) - 227742..228950 (-) 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
  PMCN07_RS01130 (PMCN07_0219) - 229010..229369 (-) 360 WP_230306568.1 hypothetical protein -
  PMCN07_RS01135 (PMCN07_0220) radC 229462..229932 (-) 471 WP_012564928.1 RadC family protein -
  PMCN07_RS01140 (PMCN07_0221) - 230071..230712 (-) 642 WP_047922032.1 DUF3560 domain-containing protein -
  PMCN07_RS01145 (PMCN07_0222) - 230827..231255 (-) 429 WP_011271832.1 STY4534 family ICE replication protein -
  PMCN07_RS01150 (PMCN07_0223) - 232140..234185 (-) 2046 WP_012564926.1 DNA topoisomerase III -
  PMCN07_RS01155 (PMCN07_0224) - 234276..234833 (+) 558 WP_012564925.1 plasmid fertility inhibition factor family protein -
  PMCN07_RS01160 (PMCN07_0225) - 235049..235567 (-) 519 WP_047922027.1 membrane lipoprotein lipid attachment site-containing protein -
  PMCN07_RS01165 (PMCN07_0226) ssb 235594..236016 (-) 423 WP_050951544.1 single-stranded DNA-binding protein Machinery gene
  PMCN07_RS01170 (PMCN07_0227) - 236263..236736 (-) 474 WP_005653583.1 DUF3158 family protein -
  PMCN07_RS01175 (PMCN07_0228) - 236751..237506 (-) 756 WP_005667775.1 PFL_4669 family integrating conjugative element protein -
  PMCN07_RS01185 (PMCN07_0230) - 237731..238954 (-) 1224 WP_012564922.1 STY4528 family pathogenicity island replication protein -
  PMCN07_RS01190 (PMCN07_0231) - 239104..239655 (-) 552 WP_076090461.1 DUF2857 domain-containing protein -
  PMCN07_RS01195 - 239655..241342 (-) 1688 Protein_228 ParB family protein -
  PMCN07_RS01200 (PMCN07_0234) dnaB 241335..242690 (-) 1356 WP_012564920.1 replicative DNA helicase -
  PMCN07_RS01205 (PMCN07_0235) - 242692..243528 (-) 837 WP_005653598.1 ParA family protein -

Sequence


Protein


Download         Length: 140 a.a.        Molecular weight: 15746.57 Da        Isoelectric Point: 5.7558

>NTDB_id=116214 PMCN07_RS01165 WP_050951544.1 235594..236016(-) (ssb) [Pasteurella multocida subsp. multocida str. HN07]
MAGINKVIIVGHLGNDPEMRSMPNGEAVANISVATSEAWTDKNTGERREVTEWHRIVFYRKLAEICGQYLKKGAQVYIEG
RLRTRKWQDQNGQDRYTTEIQGDVMQMLGTRPQSADGANNSQPMPQQDASANTFDDSIPF

Nucleotide


Download         Length: 423 bp        

>NTDB_id=116214 PMCN07_RS01165 WP_050951544.1 235594..236016(-) (ssb) [Pasteurella multocida subsp. multocida str. HN07]
ATGGCTGGAATTAATAAAGTAATTATCGTGGGACATTTAGGTAATGATCCTGAAATGCGAAGCATGCCGAATGGTGAGGC
AGTTGCAAATATCAGTGTAGCAACTAGCGAAGCTTGGACGGATAAAAACACCGGTGAACGTCGTGAAGTGACTGAATGGC
ATCGAATCGTTTTTTATCGCAAATTAGCTGAAATTTGCGGTCAATACCTCAAGAAGGGAGCTCAAGTCTATATTGAAGGT
CGCTTACGTACTCGTAAATGGCAAGATCAAAACGGGCAAGATCGTTATACCACGGAAATCCAAGGTGATGTAATGCAAAT
GTTAGGTACTCGCCCTCAAAGTGCAGATGGTGCAAATAATTCTCAGCCAATGCCACAACAAGATGCATCGGCAAATACTT
TTGATGATTCAATACCATTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A5C6GZH4

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Glaesserella parasuis strain SC1401

55.556

100

0.714

  ssb Vibrio cholerae strain A1552

52.023

100

0.643

  ssb Neisseria meningitidis MC58

59.13

82.143

0.486

  ssb Neisseria gonorrhoeae MS11

59.13

82.143

0.486


Multiple sequence alignment