Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R2K28_RS19815 Genome accession   NZ_OY734020
Coordinates   4335889..4336962 (-) Length   357 a.a.
NCBI ID   WP_116447244.1    Uniprot ID   -
Organism   Candidatus Thiodiazotropha sp. CDECU1 isolate 46184     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4330889..4341962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2K28_RS19790 aroK 4331314..4331835 (-) 522 WP_316367186.1 shikimate kinase AroK -
  R2K28_RS19795 pilQ 4332000..4334108 (-) 2109 WP_316367187.1 type IV pilus secretin PilQ -
  R2K28_RS19800 - 4334194..4334733 (-) 540 WP_116447247.1 pilus assembly protein PilP -
  R2K28_RS19805 - 4334730..4335332 (-) 603 WP_316367190.1 type 4a pilus biogenesis protein PilO -
  R2K28_RS19810 - 4335329..4335889 (-) 561 WP_116447245.1 PilN domain-containing protein -
  R2K28_RS19815 pilM 4335889..4336962 (-) 1074 WP_116447244.1 pilus assembly protein PilM Machinery gene
  R2K28_RS19820 - 4337170..4339641 (+) 2472 WP_316367192.1 penicillin-binding protein 1A -
  R2K28_RS19825 - 4339649..4340260 (+) 612 WP_316367194.1 hypothetical protein -
  R2K28_RS19830 - 4340529..4341833 (-) 1305 WP_316367195.1 citrate synthase -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 38202.13 Da        Isoelectric Point: 4.4595

>NTDB_id=1160765 R2K28_RS19815 WP_116447244.1 4335889..4336962(-) (pilM) [Candidatus Thiodiazotropha sp. CDECU1 isolate 46184]
MSLFGRKKPPLIGIDISSTAIKLLELSRATGRNGASYRVEAYGVEPLSGNAVVEKNIAEVDAVGEAIRAAVRRSGSRAKH
AAVAVSGSAVITKVISMPAALSDQELESQIQLEADQYIPYPLEEVNLDFEVIGPTEKNAELVDVLLAASRSENVDDRVAA
LELAGLSAHIVDVEAYAMENACSQLMDQLPEHGEDQTIAIADIGATTTTLNVLHNNKIIYTREQNFGGRQLTEEIQRRYG
LSIEEAGMAKRQGGLPDNYVPEVLDPFKEAMAQQVSRSLQFFFSSSSYNSVDHIILAGGSSSIPGIDDLIEEKLGTPASV
ANPFANMSVSSRVKPQALSADAPALMIACGLALRSFD

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=1160765 R2K28_RS19815 WP_116447244.1 4335889..4336962(-) (pilM) [Candidatus Thiodiazotropha sp. CDECU1 isolate 46184]
ATGTCACTATTCGGAAGAAAAAAACCGCCTTTGATAGGCATAGATATCAGCTCAACAGCCATCAAATTGCTGGAGCTGTC
CAGGGCTACCGGACGTAATGGCGCAAGCTACCGTGTGGAAGCCTATGGGGTAGAGCCCCTGTCGGGCAATGCAGTCGTCG
AGAAGAATATCGCGGAAGTCGATGCTGTCGGTGAGGCGATACGCGCAGCGGTCAGACGCTCCGGGTCGAGGGCCAAACAT
GCTGCGGTAGCGGTATCCGGCTCAGCTGTGATCACCAAGGTCATATCGATGCCGGCTGCCCTTTCTGATCAGGAGCTGGA
GAGTCAGATTCAGCTTGAAGCGGATCAGTATATTCCCTACCCACTGGAAGAGGTCAACCTGGACTTTGAGGTCATCGGCC
CAACAGAAAAAAATGCCGAGTTGGTGGATGTGCTGTTGGCCGCCTCCCGCAGCGAGAATGTTGACGATCGTGTCGCGGCA
CTGGAGTTGGCCGGCCTCTCCGCTCACATTGTGGATGTAGAGGCCTACGCCATGGAGAACGCCTGCTCCCAATTGATGGA
TCAGCTCCCCGAGCATGGCGAGGATCAAACCATCGCTATTGCGGACATTGGCGCCACCACCACCACACTGAATGTCCTGC
ACAACAACAAGATCATCTATACACGAGAACAAAATTTCGGCGGCAGGCAGCTGACGGAAGAGATACAGCGCCGCTACGGC
CTCTCTATCGAGGAGGCCGGTATGGCTAAGCGGCAGGGAGGTCTACCGGATAACTATGTACCAGAGGTACTGGATCCCTT
TAAGGAGGCCATGGCGCAACAGGTCAGCCGGTCATTGCAGTTCTTTTTCTCCTCCAGCTCCTATAATAGCGTCGATCATA
TCATTTTGGCCGGAGGCAGCTCATCCATCCCCGGCATAGACGATCTGATTGAGGAGAAATTGGGAACCCCGGCATCGGTA
GCGAATCCGTTTGCCAACATGTCGGTTTCATCCAGGGTAAAACCCCAGGCGCTGAGTGCCGATGCACCGGCACTGATGAT
TGCCTGTGGTCTGGCATTGAGGAGTTTTGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.782

100

0.538
  comM Acinetobacter nosocomialis M2

53.501

100

0.535
  comM Acinetobacter baylyi ADP1

51.821

100

0.518
  pilM Legionella pneumophila strain ERS1305867

45.658

100

0.457

Multiple sequence alignment