Detailed information NTG1160193

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QSK41_RS07935
• Genome accession: NZ_OX638701
• Coordinates: 1713293..1714441 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 4782MK isolate 4782MK
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1708293..1719441

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QSK41_RS07910	-	1709444..1709740 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QSK41_RS07915 (PAER4782_07920)	-	1709737..1710330 (-)	594	WP_003155373.1	YggT family protein	-
QSK41_RS07920 (PAER4782_07925)	proC	1710341..1711162 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QSK41_RS07925 (PAER4782_07930)	-	1711174..1711866 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QSK41_RS07930 (PAER4782_07935)	pilT	1712081..1713115 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QSK41_RS07935 (PAER4782_07940)	pilU	1713293..1714441 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QSK41_RS07940 (PAER4782_07945)	czcD	1714449..1715348 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QSK41_RS07945 (PAER4782_07950)	-	1715503..1715907 (+)	405	WP_003084560.1	NINE protein	-
QSK41_RS07950 (PAER4782_07955)	-	1716150..1717523 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QSK41_RS07955 (PAER4782_07960)	-	1717520..1718704 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1160193 QSK41_RS07935 WP_003100959.1 1713293..1714441(+) (pilU) [Pseudomonas aeruginosa strain 4782MK isolate 4782MK]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1160193 QSK41_RS07935 WP_003100959.1 1713293..1714441(+) (pilU) [Pseudomonas aeruginosa strain 4782MK isolate 4782MK]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382