Detailed information NTG1160154

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QPK16_RS01290
• Genome accession: NZ_OX638610
• Coordinates: 264988..266136 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 3541 isolate 3541
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 259988..271136

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QPK16_RS01270 (JCHGIK_02595)	-	260726..261910 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
QPK16_RS01275 (JCHGIK_02600)	-	261907..263280 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QPK16_RS01280 (JCHGIK_02605)	-	263523..263927 (-)	405	WP_003084560.1	NINE protein	-
QPK16_RS01285 (JCHGIK_02610)	czcD	264081..264980 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QPK16_RS01290 (JCHGIK_02615)	pilU	264988..266136 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QPK16_RS01295 (JCHGIK_02620)	pilT	266314..267348 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QPK16_RS01300 (JCHGIK_02625)	-	267563..268255 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QPK16_RS01305 (JCHGIK_02630)	proC	268267..269088 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QPK16_RS01310 (JCHGIK_02635)	-	269099..269692 (+)	594	WP_003084544.1	YggT family protein	-
QPK16_RS01315	-	269689..269985 (+)	297	WP_043095950.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1160154 QPK16_RS01290 WP_003100959.1 264988..266136(-) (pilU) [Pseudomonas aeruginosa strain 3541 isolate 3541]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1160154 QPK16_RS01290 WP_003100959.1 264988..266136(-) (pilU) [Pseudomonas aeruginosa strain 3541 isolate 3541]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382