Detailed information NTG1160120

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QRQ54_RS02055
• Genome accession: NZ_OX638564
• Coordinates: 443242..444390 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 3796A isolate 3796A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 438242..449390

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRQ54_RS02030	-	439393..439689 (-)	297	WP_043095950.1	DUF167 domain-containing protein	-
QRQ54_RS02035 (PAE3796A_02025)	-	439686..440279 (-)	594	WP_003084544.1	YggT family protein	-
QRQ54_RS02040 (PAE3796A_02030)	proC	440290..441111 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QRQ54_RS02045 (PAE3796A_02035)	-	441123..441815 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QRQ54_RS02050 (PAE3796A_02040)	pilT	442030..443064 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QRQ54_RS02055 (PAE3796A_02045)	pilU	443242..444390 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
QRQ54_RS02060 (PAE3796A_02050)	czcD	444398..445297 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QRQ54_RS02065 (PAE3796A_02055)	-	445451..445855 (+)	405	WP_003084560.1	NINE protein	-
QRQ54_RS02070 (PAE3796A_02060)	-	446098..447471 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
QRQ54_RS02075 (PAE3796A_02065)	-	447468..448652 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1160120 QRQ54_RS02055 WP_003100959.1 443242..444390(+) (pilU) [Pseudomonas aeruginosa strain 3796A isolate 3796A]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1160120 QRQ54_RS02055 WP_003100959.1 443242..444390(+) (pilU) [Pseudomonas aeruginosa strain 3796A isolate 3796A]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382