Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RG36_RS13455 Genome accession   NZ_CP010140
Coordinates   2545306..2546316 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain D3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2540306..2551316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG36_RS13435 (RG36_12660) mepM 2541079..2542401 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  RG36_RS13440 (RG36_12665) znuA 2542417..2543349 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  RG36_RS13445 (RG36_12670) znuC 2543428..2544183 (+) 756 WP_000202986.1 zinc ABC transporter ATP-binding protein ZnuC -
  RG36_RS13450 (RG36_12675) znuB 2544180..2544965 (+) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  RG36_RS13455 (RG36_12680) ruvB 2545306..2546316 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RG36_RS13460 (RG36_12685) ruvA 2546325..2546936 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  RG36_RS13465 yobI 2547075..2547140 (-) 66 WP_072161512.1 stress response small protein YobI -
  RG36_RS13470 (RG36_12690) yebB 2547211..2547813 (+) 603 WP_001024912.1 YebB family permuted papain-like enzyme -
  RG36_RS13475 (RG36_12695) ruvC 2547815..2548336 (-) 522 WP_001324008.1 crossover junction endodeoxyribonuclease RuvC -
  RG36_RS13480 (RG36_12700) yebC 2548371..2549111 (-) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RG36_RS13485 (RG36_12705) nudB 2549140..2549592 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=116004 RG36_RS13455 WP_000568519.1 2545306..2546316(-) (ruvB) [Escherichia coli strain D3]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=116004 RG36_RS13455 WP_000568519.1 2545306..2546316(-) (ruvB) [Escherichia coli strain D3]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACACGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCTGAAGGTTTTGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAATTCTTTGGTGGGCCTGTAGGTCTGGATAACCTGGCGGCGGCTATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAGCCTTATCTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment