Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   QOS64_RS18350 Genome accession   NZ_OX460973
Coordinates   3655406..3656791 (-) Length   461 a.a.
NCBI ID   WP_168984791.1    Uniprot ID   -
Organism   Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3650406..3661791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOS64_RS18315 flgL 3650581..3651498 (-) 918 WP_007407477.1 flagellar hook-associated protein FlgL -
  QOS64_RS18320 flgK 3651510..3653027 (-) 1518 WP_007407478.1 flagellar hook-associated protein FlgK -
  QOS64_RS18325 - 3653043..3653525 (-) 483 WP_003151394.1 flagellar protein FlgN -
  QOS64_RS18330 flgM 3653540..3653806 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  QOS64_RS18335 - 3653876..3654295 (-) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  QOS64_RS18340 comFC 3654369..3655058 (-) 690 WP_219152091.1 phosphoribosyltransferase family protein Machinery gene
  QOS64_RS18345 - 3655064..3655348 (-) 285 WP_007407481.1 competence protein ComFB -
  QOS64_RS18350 comFA 3655406..3656791 (-) 1386 WP_168984791.1 DEAD/DEAH box helicase Machinery gene
  QOS64_RS18355 - 3656899..3657747 (-) 849 WP_007614105.1 DegV family protein -
  QOS64_RS18360 degU 3657845..3658534 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  QOS64_RS18365 degS 3658611..3659774 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  QOS64_RS18370 - 3659997..3660644 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52574.88 Da        Isoelectric Point: 10.1584

>NTDB_id=1159475 QOS64_RS18350 WP_168984791.1 3655406..3656791(-) (comFA) [Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAHENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVDVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNVRAEGVHAEDTFRKDKVKRFRDGQLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKYFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1159475 QOS64_RS18350 WP_168984791.1 3655406..3656791(-) (comFA) [Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAAGCGTTTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCTTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCATGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCTCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGACGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACCGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACATGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAATGTAAGGGCAGAAGGCGTTCATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCAACTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAATATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(328-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

63.261

99.783

0.631
  comFA Latilactobacillus sakei subsp. sakei 23K

40.097

89.805

0.36

Multiple sequence alignment