Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   KJP52_RS21355 Genome accession   NZ_OX419575
Coordinates   4106729..4107247 (-) Length   172 a.a.
NCBI ID   WP_003219228.1    Uniprot ID   A0A063XE16
Organism   Bacillus subtilis isolate NRS6121     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 4057135..4106714 4106729..4107247 flank 15


Gene organization within MGE regions


Location: 4057135..4107247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP52_RS21020 (NRS6121_20770) yybO 4057550..4058857 (+) 1308 WP_009968439.1 MFS transporter -
  KJP52_RS21025 (NRS6121_20775) - 4058901..4059140 (-) 240 WP_003244542.1 hypothetical protein -
  KJP52_RS21030 - 4059184..4059273 (-) 90 Protein_4086 hypothetical protein -
  KJP52_RS21035 - 4059306..4059491 (-) 186 WP_009968440.1 hypothetical protein -
  KJP52_RS21040 (NRS6121_20780) yybN 4060619..4061056 (+) 438 WP_009968442.1 DUF2712 domain-containing protein -
  KJP52_RS21045 (NRS6121_20785) yybM 4061170..4061925 (+) 756 WP_003244187.1 DUF2705 family protein -
  KJP52_RS21050 (NRS6121_20790) yybL 4061915..4062625 (+) 711 WP_003243512.1 WxPxxD family membrane protein -
  KJP52_RS21055 (NRS6121_20795) yybK 4062622..4063377 (+) 756 WP_003243304.1 DUF2705 family protein -
  KJP52_RS21060 (NRS6121_20800) yybJ 4063374..4064030 (+) 657 WP_003243153.1 ATP-binding cassette domain-containing protein -
  KJP52_RS21065 - 4064182..4064359 (+) 178 Protein_4093 MFS transporter -
  KJP52_RS21070 (NRS6121_20805) - 4064405..4065193 (-) 789 WP_003244492.1 hypothetical protein -
  KJP52_RS21075 (NRS6121_20810) yybH 4065261..4065650 (-) 390 WP_003243451.1 nuclear transport factor 2 family protein -
  KJP52_RS21080 (NRS6121_20815) yybG 4065796..4066635 (+) 840 WP_003244562.1 pentapeptide repeat-containing protein -
  KJP52_RS21085 (NRS6121_20820) yybF 4066668..4067882 (-) 1215 WP_003243239.1 MFS transporter -
  KJP52_RS21090 (NRS6121_20825) yybE 4068069..4068947 (+) 879 WP_003242958.1 LysR family transcriptional regulator -
  KJP52_RS21095 (NRS6121_20830) yybD 4068961..4069404 (+) 444 WP_003226881.1 GNAT family N-acetyltransferase -
  KJP52_RS21100 (NRS6121_20835) yybC 4069487..4069966 (+) 480 WP_003243923.1 hypothetical protein -
  KJP52_RS21105 - 4069991..4070124 (-) 134 Protein_4101 LysR family transcriptional regulator -
  KJP52_RS21110 (NRS6121_20840) yybB 4070141..4070803 (-) 663 WP_003226877.1 MBL fold metallo-hydrolase -
  KJP52_RS21115 (NRS6121_20845) yybA 4070950..4071402 (-) 453 WP_003226875.1 MarR family transcriptional regulator -
  KJP52_RS21120 (NRS6121_20850) yyaT 4071522..4071968 (+) 447 WP_003243577.1 GNAT family N-acetyltransferase -
  KJP52_RS21125 (NRS6121_20855) yyaS 4071965..4072570 (+) 606 WP_003244154.1 YitT family protein -
  KJP52_RS21130 (NRS6121_20860) satA 4072665..4073186 (-) 522 WP_003242546.1 streptothricin N-acetyltransferase SatA -
  KJP52_RS21135 (NRS6121_20865) yyaQ 4073597..4073953 (+) 357 WP_003244448.1 MmcQ/YjbR family DNA-binding protein -
  KJP52_RS21140 (NRS6121_20870) yyaP 4074113..4074679 (+) 567 WP_003243497.1 dihydrofolate reductase family protein -
  KJP52_RS21145 - 4074921..4075083 (+) 163 Protein_4109 DUF255 domain-containing protein -
  KJP52_RS21150 (NRS6121_20875) tet(L) 4075186..4076562 (-) 1377 WP_003242953.1 tetracycline efflux MFS transporter Tet(L) -
  KJP52_RS21155 (NRS6121_20885) - 4076911..4077150 (+) 240 WP_003243894.1 DUF255 domain-containing protein -
  KJP52_RS21160 (NRS6121_20890) yyaN 4077301..4077717 (+) 417 WP_010886646.1 MerR family transcriptional regulator -
  KJP52_RS21165 (NRS6121_20895) yyaM 4077714..4078631 (+) 918 WP_003242776.1 EamA family transporter -
  KJP52_RS21170 (NRS6121_20900) - 4078703..4078903 (+) 201 WP_213384499.1 DUF255 domain-containing protein -
  KJP52_RS21175 (NRS6121_20905) - 4078959..4080467 (+) 1509 WP_213384498.1 recombinase family protein -
  KJP52_RS21180 (NRS6121_20910) - 4080544..4080930 (-) 387 WP_213384497.1 cystatin-like fold lipoprotein -
  KJP52_RS21185 (NRS6121_20915) - 4080984..4081490 (-) 507 WP_213384496.1 hypothetical protein -
  KJP52_RS21190 (NRS6121_20920) cwlT 4081505..4082494 (-) 990 WP_087961683.1 bifunctional lytic transglycosylase/C40 family peptidase -
  KJP52_RS21195 (NRS6121_20925) - 4082491..4084941 (-) 2451 WP_213384495.1 hypothetical protein -
  KJP52_RS21200 (NRS6121_20930) yddF 4084945..4085271 (-) 327 WP_049635835.1 YddF family protein -
  KJP52_RS21205 (NRS6121_20935) conE 4085289..4087784 (-) 2496 WP_213384494.1 ATP-binding protein -
  KJP52_RS21210 (NRS6121_20940) conD 4087672..4088196 (-) 525 WP_072174271.1 conjugal transfer protein -
  KJP52_RS21215 (NRS6121_20945) conC 4088209..4088457 (-) 249 WP_014478915.1 transposon conjugation system subunit ConC -
  KJP52_RS21220 (NRS6121_20950) conB 4088470..4089534 (-) 1065 WP_213384493.1 conjugal transfer protein -
  KJP52_RS21225 (NRS6121_20955) - 4089562..4089711 (-) 150 WP_003328171.1 hypothetical protein -
  KJP52_RS21230 (NRS6121_20960) - 4089729..4090007 (-) 279 WP_082170512.1 hypothetical protein -
  KJP52_RS21235 (NRS6121_20965) - 4090020..4090337 (-) 318 WP_213384492.1 hypothetical protein -
  KJP52_RS21240 (NRS6121_20970) - 4090349..4090615 (-) 267 WP_129093540.1 hypothetical protein -
  KJP52_RS21245 (NRS6121_20975) - 4090608..4090859 (-) 252 WP_014478910.1 DUF3850 domain-containing protein -
  KJP52_RS21250 (NRS6121_20985) nicK 4091130..4092188 (-) 1059 WP_213384491.1 replication initiation factor domain-containing protein -
  KJP52_RS21255 (NRS6121_20990) - 4092181..4092987 (-) 807 Protein_4131 DUF87 domain-containing protein -
  KJP52_RS21260 (NRS6121_20995) - 4092995..4094404 (-) 1410 WP_213384484.1 ATP-binding protein -
  KJP52_RS21265 (NRS6121_21000) - 4094436..4094675 (-) 240 WP_213384483.1 hypothetical protein -
  KJP52_RS21270 (NRS6121_21005) helP 4094705..4095085 (-) 381 WP_213384481.1 YdcP family protein -
  KJP52_RS21275 - 4095102..4095248 (-) 147 WP_213384480.1 hypothetical protein -
  KJP52_RS21280 (NRS6121_21010) - 4095450..4095710 (-) 261 WP_213384479.1 hypothetical protein -
  KJP52_RS21285 - 4095764..4096024 (-) 261 WP_213384478.1 hypothetical protein -
  KJP52_RS21290 (NRS6121_21015) - 4096021..4096200 (-) 180 WP_017418449.1 hypothetical protein -
  KJP52_RS21295 (NRS6121_21020) - 4096495..4096878 (+) 384 WP_017418448.1 helix-turn-helix transcriptional regulator -
  KJP52_RS21300 (NRS6121_21025) - 4096875..4097405 (+) 531 WP_213384476.1 ImmA/IrrE family metallo-endopeptidase -
  KJP52_RS21305 (NRS6121_21035) - 4097517..4098731 (-) 1215 WP_026092273.1 Rap family tetratricopeptide repeat protein -
  KJP52_RS21310 (NRS6121_21040) - 4099021..4099182 (+) 162 WP_077199439.1 DUF255 domain-containing protein -
  KJP52_RS21315 (NRS6121_21045) yyaL 4099221..4101110 (+) 1890 WP_082252133.1 thioredoxin domain-containing protein -
  KJP52_RS21320 (NRS6121_21050) yyaK 4101107..4102006 (-) 900 WP_003244361.1 type II CAAX endopeptidase family protein -
  KJP52_RS21325 (NRS6121_21055) yyaJ 4102232..4103587 (+) 1356 WP_003243011.1 MFS transporter -
  KJP52_RS21330 (NRS6121_21060) maa 4103621..4104175 (-) 555 WP_003242928.1 sugar O-acetyltransferase -
  KJP52_RS21335 (NRS6121_21065) yyaH 4104193..4104573 (-) 381 WP_003244460.1 VOC family protein -
  KJP52_RS21340 (NRS6121_21070) ccpB 4104629..4105564 (-) 936 WP_003243573.1 transcriptional regulator CcpB -
  KJP52_RS21345 (NRS6121_21075) xth 4105623..4106381 (-) 759 WP_003243194.1 exodeoxyribonuclease III -
  KJP52_RS21350 (NRS6121_21080) rpsR 4106446..4106685 (-) 240 WP_003219224.1 30S ribosomal protein S18 -
  KJP52_RS21355 (NRS6121_21085) ssbA 4106729..4107247 (-) 519 WP_003219228.1 single-stranded DNA-binding protein SsbA Machinery gene

Sequence


Protein


Download         Length: 172 a.a.        Molecular weight: 18742.31 Da        Isoelectric Point: 4.7621

>NTDB_id=1157626 KJP52_RS21355 WP_003219228.1 4106729..4107247(-) (ssbA) [Bacillus subtilis isolate NRS6121]
MLNRVVLVGRLTKDPELRYTPNGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNGGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=1157626 KJP52_RS21355 WP_003219228.1 4106729..4107247(-) (ssbA) [Bacillus subtilis isolate NRS6121]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAACGGTGCGGCTGTTGC
TACGTTTACTCTTGCTGTGAATCGTACATTTACGAACCAGTCCGGAGAACGTGAGGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTCTTGAAAAAAGGAAGCCTTGCAGGCGTAGATGGCCGTTTACAAACAAGA
AACTATGAAAACCAGCAAGGACAGCGTGTCTTCGTGACAGAGGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCGAAAAA
CGGCGGCGGTTCTGGTTCAGGTGGATACAACGAAGGAAACAGCGGCGGAGGCCAGTACTTTGGCGGAGGCCAAAATGATA
ATCCATTTGGGGGAAATCAAAACAACCAGAGACGCAATCAGGGGAACAGCTTTAATGATGACCCATTTGCCAACGACGGC
AAACCGATTGACATCTCGGATGATGATCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A063XE16

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

100

100

1

  ssb Latilactobacillus sakei subsp. sakei 23K

58.192

100

0.599

  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

61.628

0.395

  ssb Glaesserella parasuis strain SC1401

35.519

100

0.378


Multiple sequence alignment