Detailed information NTG0115274

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: B824_RS08730
• Genome accession: NZ_CP006957
• Coordinates: 1779606..1780919 (+)
• Length: 437 a.a.
• NCBI ID: WP_040080997.1
• Uniprot ID: -
• Organism: Mannheimia haemolytica USDA-ARS-USMARC-184
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1774606..1785919

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B824_RS08705 (B824_18620)	-	1774769..1777333 (-)	2565	WP_021280120.1	penicillin-binding protein 1A	-
B824_RS08710 (B824_18630)	-	1777448..1778146 (+)	699	WP_006247933.1	hypothetical protein	-
B824_RS08715 (B824_18640)	-	1778122..1778643 (+)	522	WP_040080995.1	hypothetical protein	-
B824_RS08720 (B824_18650)	-	1778645..1779181 (+)	537	WP_006247935.1	hypothetical protein	-
B824_RS08725 (B824_18660)	-	1779184..1779597 (+)	414	WP_040080996.1	hypothetical protein	-
B824_RS08730 (B824_18670)	comE	1779606..1780919 (+)	1314	WP_040080997.1	type IV pilus secretin PilQ	Machinery gene
B824_RS08735 (B824_18680)	nusB	1781000..1781413 (+)	414	WP_006247938.1	transcription antitermination factor NusB	-
B824_RS08740 (B824_18690)	thiL	1781476..1782456 (+)	981	WP_006247939.1	thiamine-phosphate kinase	-
B824_RS08745 (B824_18700)	-	1782461..1782934 (+)	474	WP_006247940.1	phosphatidylglycerophosphatase A	-
B824_RS08750 (B824_18710)	-	1782941..1783564 (+)	624	WP_006247941.1	LysE family transporter	-
B824_RS08755 (B824_18720)	ruvA	1783676..1784281 (+)	606	WP_006247943.1	Holliday junction branch migration protein RuvA	-
B824_RS08760 (B824_18730)	ruvB	1784418..1785422 (+)	1005	WP_040080998.1	Holliday junction branch migration DNA helicase RuvB	-

Sequence

Protein

• Length: 437 a.a.
• Molecular weight: 48487.52 Da
• Isoelectric Point: 8.0673

>NTDB_id=115274 B824_RS08730 WP_040080997.1 1779606..1780919(+) (comE) [Mannheimia haemolytica USDA-ARS-USMARC-184]
MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNDVTNNLNVN
FPITGGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK

Nucleotide

• Length: 1314 bp

>NTDB_id=115274 B824_RS08730 WP_040080997.1 1779606..1780919(+) (comE) [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTAAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAAAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACGATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTGCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCGCAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (267-424)

• Bacterial type II/III secretion system short domain (Secretin_N) (122-187)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Glaesserella parasuis strain SC1401	63.073	99.771	0.629
comE	Haemophilus influenzae Rd KW20	51.034	99.542	0.508
comE	Haemophilus influenzae 86-028NP	50.345	99.542	0.501
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	39.192	96.339	0.378
pilQ	Vibrio cholerae strain A1552	39.192	96.339	0.378
pilQ	Vibrio campbellii strain DS40M4	37.355	98.627	0.368