Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   F544_RS08870 Genome accession   NZ_CP006956
Coordinates   1945309..1946568 (+) Length   419 a.a.
NCBI ID   WP_015433224.1    Uniprot ID   -
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-190     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1940309..1951568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F544_RS08845 (F544_17860) - 1940435..1943047 (-) 2613 WP_025289744.1 penicillin-binding protein 1A -
  F544_RS08850 (F544_17870) - 1943213..1943848 (+) 636 WP_025289745.1 hypothetical protein -
  F544_RS11445 (F544_17880) - 1943830..1944357 (+) 528 WP_015433221.1 hypothetical protein -
  F544_RS08860 (F544_17890) - 1944357..1944872 (+) 516 WP_025289746.1 hypothetical protein -
  F544_RS08865 (F544_17900) - 1944881..1945300 (+) 420 WP_025289747.1 hypothetical protein -
  F544_RS08870 (F544_17910) comE 1945309..1946568 (+) 1260 WP_015433224.1 type IV pilus secretin PilQ Machinery gene
  F544_RS08875 (F544_17920) nusB 1946721..1947146 (+) 426 WP_015433225.1 transcription antitermination factor NusB -
  F544_RS08880 (F544_17930) thiL 1947162..1948130 (+) 969 WP_025289748.1 thiamine-phosphate kinase -
  F544_RS08885 (F544_17940) - 1948133..1948606 (+) 474 WP_025266803.1 phosphatidylglycerophosphatase A -
  F544_RS08890 (F544_17950) - 1948628..1949254 (+) 627 WP_025289749.1 LysE family transporter -
  F544_RS08895 (F544_17960) modF 1949255..1950718 (+) 1464 WP_025289750.1 molybdate ABC transporter ATP-binding protein ModF -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 46729.73 Da        Isoelectric Point: 8.3598

>NTDB_id=115251 F544_RS08870 WP_015433224.1 1945309..1946568(+) (comE) [Bibersteinia trehalosi USDA-ARS-USMARC-190]
MRYILLLFSTMISFTMAQTISLNLKNAPTAEIISYLAEESGKNVVLSDNLSDTKSLRLENFTFEQILQSLAKLHQFSLKQ
EKGIYYISEKPLEPAKANPIASITPKMPKLITKTIKLHYAKASEVIHSLTQGSGTMLAENGYIHFDERSNSVIIKDTATS
IKNLRALIAQLDQPTEQIAIEARIVTINSENLQELGVRWGMFSPNTHHHRFSGNLEGSGLPMNNLNVNFPVNNAASMVLQ
VASINSRVLDLELTALERENSVEIIASPRLLTTNKKSANIKQGTEIPYAVYSQKDELRNIEFREAVLGLEVTPHLSKNNQ
ILLDLVVSQNSPNSQTTGSDMVTIDKQEINTQVFAKHGETIVLGGIFQHLIAKGEDRVPILGSIPVIKRLFSQSRDKIAK
RELVIFVTPYIVKSERIEN

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=115251 F544_RS08870 WP_015433224.1 1945309..1946568(+) (comE) [Bibersteinia trehalosi USDA-ARS-USMARC-190]
ATGCGTTATATTCTTTTGTTATTTAGCACCATGATTTCCTTTACCATGGCACAAACAATCAGTTTAAATTTAAAAAATGC
GCCTACCGCAGAAATCATTAGTTATCTAGCGGAAGAAAGTGGCAAAAATGTAGTGCTATCGGATAATTTAAGTGACACAA
AATCACTTAGATTAGAAAATTTCACATTTGAGCAAATTCTGCAAAGTTTAGCCAAATTACATCAATTTTCACTAAAACAG
GAAAAGGGCATTTATTATATTAGTGAAAAACCATTAGAACCTGCGAAAGCAAATCCTATTGCTAGCATCACTCCTAAAAT
GCCCAAACTCATCACTAAAACGATTAAATTGCATTATGCTAAAGCTTCAGAAGTCATTCATTCGCTCACACAAGGTAGTG
GCACAATGCTTGCTGAAAATGGCTATATTCACTTTGATGAACGCAGTAACAGTGTGATTATCAAAGACACCGCCACATCA
ATTAAAAATCTTCGAGCTTTAATTGCACAACTTGACCAACCAACAGAACAAATTGCAATTGAAGCTCGTATCGTTACCAT
CAATAGTGAAAATTTACAAGAACTGGGCGTTCGTTGGGGAATGTTTTCGCCTAATACTCATCATCATAGATTCTCAGGAA
ATCTTGAAGGTAGTGGTTTGCCAATGAATAACTTAAATGTCAATTTTCCTGTTAATAATGCCGCTTCGATGGTGCTTCAA
GTTGCAAGTATCAATAGCCGTGTTTTAGATTTAGAGCTCACCGCGCTTGAACGAGAAAATAGCGTTGAAATTATTGCCAG
CCCAAGATTACTTACAACGAACAAAAAAAGTGCCAACATTAAACAAGGCACAGAGATCCCTTATGCAGTCTATAGTCAAA
AAGACGAACTGAGAAATATCGAATTTCGGGAAGCGGTTTTAGGCTTGGAAGTGACTCCTCATCTTTCTAAGAATAACCAA
ATTCTGCTGGATTTAGTCGTGAGCCAAAATTCACCCAATAGCCAGACAACAGGCAGTGATATGGTAACAATTGATAAACA
AGAAATTAATACTCAGGTATTTGCAAAACATGGAGAAACCATTGTTTTAGGTGGAATTTTCCAGCATCTCATCGCAAAAG
GTGAAGATCGCGTACCGATCTTAGGTTCAATTCCAGTGATCAAGCGCCTATTTAGCCAAAGTCGCGATAAAATCGCTAAA
CGTGAATTAGTCATTTTTGTCACCCCTTATATTGTGAAATCGGAGAGAATTGAGAATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(255-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

70.264

99.523

0.699
  comE Haemophilus influenzae Rd KW20

53.428

100

0.539
  comE Haemophilus influenzae 86-028NP

52.482

100

0.53
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.423

99.284

0.391
  pilQ Vibrio cholerae strain A1552

39.423

99.284

0.391
  pilQ Vibrio campbellii strain DS40M4

37.589

100

0.379

Multiple sequence alignment