Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   F543_RS02460 Genome accession   NZ_CP006955
Coordinates   484966..486225 (-) Length   419 a.a.
NCBI ID   WP_015433224.1    Uniprot ID   -
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-189     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479966..491225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F543_RS02435 (F543_5070) modF 480908..482370 (-) 1463 Protein_482 molybdate ABC transporter ATP-binding protein ModF -
  F543_RS02440 (F543_5080) - 482371..482997 (-) 627 WP_015433228.1 LysE family transporter -
  F543_RS02445 (F543_5090) - 483019..483492 (-) 474 WP_025266803.1 phosphatidylglycerophosphatase A -
  F543_RS02450 (F543_5100) thiL 483495..484463 (-) 969 WP_015433226.1 thiamine-phosphate kinase -
  F543_RS02455 (F543_5110) nusB 484479..484904 (-) 426 WP_015433225.1 transcription antitermination factor NusB -
  F543_RS02460 (F543_5120) comE 484966..486225 (-) 1260 WP_015433224.1 type IV pilus secretin PilQ Machinery gene
  F543_RS02465 (F543_5130) - 486234..486653 (-) 420 WP_015433223.1 hypothetical protein -
  F543_RS02470 (F543_5140) - 486662..487177 (-) 516 WP_015433222.1 hypothetical protein -
  F543_RS11530 (F543_5150) - 487177..487704 (-) 528 WP_015433221.1 hypothetical protein -
  F543_RS02480 (F543_5160) - 487686..488321 (-) 636 WP_025328921.1 hypothetical protein -
  F543_RS02485 (F543_5170) - 488487..491099 (+) 2613 WP_025289744.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 46729.73 Da        Isoelectric Point: 8.3598

>NTDB_id=115219 F543_RS02460 WP_015433224.1 484966..486225(-) (comE) [Bibersteinia trehalosi USDA-ARS-USMARC-189]
MRYILLLFSTMISFTMAQTISLNLKNAPTAEIISYLAEESGKNVVLSDNLSDTKSLRLENFTFEQILQSLAKLHQFSLKQ
EKGIYYISEKPLEPAKANPIASITPKMPKLITKTIKLHYAKASEVIHSLTQGSGTMLAENGYIHFDERSNSVIIKDTATS
IKNLRALIAQLDQPTEQIAIEARIVTINSENLQELGVRWGMFSPNTHHHRFSGNLEGSGLPMNNLNVNFPVNNAASMVLQ
VASINSRVLDLELTALERENSVEIIASPRLLTTNKKSANIKQGTEIPYAVYSQKDELRNIEFREAVLGLEVTPHLSKNNQ
ILLDLVVSQNSPNSQTTGSDMVTIDKQEINTQVFAKHGETIVLGGIFQHLIAKGEDRVPILGSIPVIKRLFSQSRDKIAK
RELVIFVTPYIVKSERIEN

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=115219 F543_RS02460 WP_015433224.1 484966..486225(-) (comE) [Bibersteinia trehalosi USDA-ARS-USMARC-189]
ATGCGTTATATTCTTTTGTTATTTAGCACCATGATTTCCTTTACTATGGCACAAACAATCAGTTTAAATTTAAAAAATGC
GCCTACCGCAGAAATCATTAGTTATCTAGCGGAAGAAAGTGGCAAAAATGTAGTGCTATCGGATAATTTAAGTGACACAA
AATCACTTAGATTAGAAAATTTCACATTCGAGCAAATTCTGCAAAGTTTAGCCAAATTACATCAATTTTCACTAAAACAG
GAAAAGGGCATTTATTATATTAGTGAAAAACCATTAGAACCTGCGAAAGCAAATCCTATTGCTAGCATCACTCCTAAAAT
GCCCAAACTCATCACTAAAACGATTAAATTGCATTATGCTAAAGCTTCAGAAGTCATTCATTCGCTCACACAAGGTAGTG
GCACAATGCTTGCTGAAAATGGCTATATTCACTTTGATGAACGCAGTAACAGTGTGATTATCAAAGACACTGCCACATCA
ATTAAAAATCTTCGAGCTTTAATTGCACAACTTGACCAACCAACAGAACAAATTGCAATTGAAGCTCGTATCGTTACCAT
CAATAGTGAAAATTTACAAGAACTGGGCGTTCGTTGGGGAATGTTTTCGCCTAATACTCATCATCATAGATTCTCAGGAA
ATCTTGAAGGTAGTGGTTTGCCAATGAATAACTTAAATGTCAATTTTCCTGTTAATAATGCCGCTTCGATGGTGCTTCAA
GTTGCAAGTATCAATAGCCGTGTTTTAGATTTAGAGCTCACCGCGCTTGAACGAGAAAATAGCGTTGAAATTATTGCCAG
CCCAAGATTACTTACAACGAACAAAAAAAGTGCCAACATTAAACAAGGCACAGAGATCCCTTATGCAGTCTATAGTCAAA
AAGACGAACTGAGAAATATCGAATTTCGGGAAGCGGTTTTAGGCTTGGAAGTGACACCTCATCTTTCTAAGAATAACCAA
ATTCTGCTGGATTTAGTCGTGAGCCAAAATTCACCCAATAGCCAGACAACAGGCAGTGATATGGTAACAATTGATAAACA
AGAAATTAATACTCAGGTATTTGCAAAACATGGAGAAACCATTGTTTTAGGTGGAATTTTCCAGCATCTCATCGCAAAAG
GTGAAGATCGCGTACCGATCTTAGGTTCAATTCCAGTGATCAAGCGCCTATTTAGCCAAAGTCGCGATAAAATCGCTAAA
CGTGAATTAGTCATTTTTGTCACCCCTTATATTGTGAAATCGGAGAGAATTGAGAATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(255-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

70.264

99.523

0.699
  comE Haemophilus influenzae Rd KW20

53.428

100

0.539
  comE Haemophilus influenzae 86-028NP

52.482

100

0.53
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.423

99.284

0.391
  pilQ Vibrio cholerae strain A1552

39.423

99.284

0.391
  pilQ Vibrio campbellii strain DS40M4

37.589

100

0.379

Multiple sequence alignment