Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   X875_RS07330 Genome accession   NZ_CP006953
Coordinates   1552139..1553452 (+) Length   437 a.a.
NCBI ID   WP_025342840.1    Uniprot ID   -
Organism   Mannheimia varigena USDA-ARS-USMARC-1388     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1547139..1558452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  X875_RS07305 (X875_14580) - 1547304..1549856 (-) 2553 WP_025342836.1 penicillin-binding protein 1A -
  X875_RS07310 (X875_14590) - 1549982..1550683 (+) 702 WP_038643722.1 hypothetical protein -
  X875_RS07315 (X875_14600) - 1550659..1551180 (+) 522 WP_025342837.1 hypothetical protein -
  X875_RS07320 (X875_14610) - 1551182..1551715 (+) 534 WP_025342838.1 hypothetical protein -
  X875_RS07325 (X875_14620) - 1551717..1552130 (+) 414 WP_025342839.1 hypothetical protein -
  X875_RS07330 (X875_14630) comE 1552139..1553452 (+) 1314 WP_025342840.1 type IV pilus secretin PilQ Machinery gene
  X875_RS07335 (X875_14640) nusB 1553532..1553945 (+) 414 WP_025342841.1 transcription antitermination factor NusB -
  X875_RS07340 (X875_14650) thiL 1554008..1554988 (+) 981 WP_025342842.1 thiamine-phosphate kinase -
  X875_RS07345 (X875_14660) - 1554994..1555467 (+) 474 WP_025247432.1 phosphatidylglycerophosphatase A -
  X875_RS07350 (X875_14670) - 1555469..1556095 (+) 627 WP_025247431.1 LysE family transporter -
  X875_RS07355 (X875_14680) ruvA 1556204..1556809 (+) 606 WP_025342843.1 Holliday junction branch migration protein RuvA -
  X875_RS07360 (X875_14690) ruvB 1556820..1557824 (+) 1005 WP_025216875.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48393.48 Da        Isoelectric Point: 9.4349

>NTDB_id=115175 X875_RS07330 WP_025342840.1 1552139..1553452(+) (comE) [Mannheimia varigena USDA-ARS-USMARC-1388]
MFRIFLYLLLSSSLALADTISLAVRNAPTSLLLTYLAEETGKNIVLDDDIQTKSTLRLENKSINEIFKTLSKVNKLSFTQ
EADIVYISKKEEKSANLNSVSIAHLQNNIQNPPLVTSPKLITKTIKLNYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSAKSLQNIERLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSRGTNHYKFGGRLEGNGLSNVANNLNVN
FPVANGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNTQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKVRGMKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=115175 X875_RS07330 WP_025342840.1 1552139..1553452(+) (comE) [Mannheimia varigena USDA-ARS-USMARC-1388]
ATGTTTAGAATTTTTCTGTATTTATTACTTAGTTCGTCATTAGCATTGGCTGACACTATTTCCCTTGCAGTAAGGAATGC
ACCAACTTCACTTCTGCTAACCTATTTAGCTGAAGAAACAGGTAAAAATATAGTATTAGATGATGATATTCAAACTAAAT
CCACCTTAAGATTAGAAAATAAATCCATTAATGAGATTTTTAAAACATTGAGCAAAGTAAATAAATTATCTTTTACTCAA
GAAGCAGACATTGTCTATATTAGTAAAAAAGAAGAAAAATCGGCTAATTTAAATTCAGTTTCAATTGCCCACTTGCAAAA
TAATATACAAAACCCACCGCTTGTAACCTCTCCAAAACTGATAACTAAAACCATAAAACTAAATTATGCTAAAGCCTCAG
AAGTGATTGAATCTTTAACTAAAGGCAGTGGCACGTTTTTATCTGAAAATGGTTATATCCATTTTGACGAGCGAAGTAAT
AGCTTAATTATCAAAGATAGTGCGAAATCACTACAAAATATTGAGCGATTAGTCAAACAACTCGACCAACCCACCGAACA
AATTGCGATTGAAGCTCGCATTGTCACGATTAGTAGCGAACATTTGCAAGAATTAGGTGTGCGTTGGGGAATGTTTTCCA
GAGGAACTAATCACTATAAATTTGGTGGGCGATTGGAAGGAAATGGGTTAAGCAATGTAGCTAATAATCTCAATGTGAAT
TTCCCCGTTGCGAATGGTGCTTCAGCAGTTTTACAAGTAGCTTCCATTAATAGTCGAGTACTAGATTTAGAACTCAGTGC
ATTAGAACAGGAAAACAGTGTGGAAATCATTGCTAGCCCTCGTCTTTTAACCACTAATAAAAAACCTGCGAGTATCAAGC
AAGGCACGGAAATTCCTTATGTAATGTATAACACCAAATCAGAAGCAACCGATGTCGAATTTAAAGAAGCAGTGTTAGGA
TTGGAAGTTGTTCCTCATCTTTCTACTCAAAATCAAATTTTGTTGGATTTAATCGTCACTCAAAACTCACCGAATACACA
ATCTGGTAGCAGTGGTTTAATTACCATTGATAAGCAAGAACTTAATACACAGGTGTTTGCTAAACACGGTGAAACTATCG
TGCTAGGTGGTATTTTCCAACATTTAACCCAAAAAGGAGAAGATAAAGTACCTATTTTAGGCTCAATTCCGTTTATTAAA
CGATTATTTAGCCAAACAAGAGACAAAATCAGCAAGCGGGAACTTGTTATTTTTGTTACCCCTTACATTCTCCAATCAAA
CGAAAAAGTAAGGGGCATGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.678

99.542

0.634
  comE Haemophilus influenzae 86-028NP

48.352

100

0.503
  comE Haemophilus influenzae Rd KW20

51.319

95.423

0.49
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.81

96.568

0.384
  pilQ Vibrio cholerae strain A1552

39.81

96.568

0.384
  pilQ Vibrio campbellii strain DS40M4

37.995

98.169

0.373

Multiple sequence alignment