Detailed information NTG0115172

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: X875_RS06365
• Genome accession: NZ_CP006953
• Coordinates: 1348834..1350030 (-)
• Length: 398 a.a.
• NCBI ID: WP_025342724.1
• Uniprot ID: -
• Organism: Mannheimia varigena USDA-ARS-USMARC-1388
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1343834..1355030

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
X875_RS06340 (X875_12570)	lptE	1345789..1346277 (+)	489	WP_025342720.1	LPS assembly lipoprotein LptE	-
X875_RS06345 (X875_12580)	holA	1346317..1347339 (+)	1023	WP_025342721.1	DNA polymerase III subunit delta	-
X875_RS06350 (X875_12590)	yacG	1347340..1347525 (-)	186	WP_025217069.1	DNA gyrase inhibitor YacG	-
X875_RS06355 (X875_12600)	coaE	1347507..1348142 (-)	636	WP_025342722.1	dephospho-CoA kinase	-
X875_RS06360 (X875_12610)	-	1348174..1348830 (-)	657	WP_025342723.1	prepilin peptidase	-
X875_RS06365 (X875_12620)	pilC	1348834..1350030 (-)	1197	WP_025342724.1	type II secretion system F family protein	Machinery gene
X875_RS06370 (X875_12630)	pilB	1350023..1351414 (-)	1392	WP_025342725.1	GspE/PulE family protein	Machinery gene
X875_RS06375 (X875_12640)	pilA	1351435..1351914 (-)	480	WP_025217064.1	prepilin peptidase-dependent pilin	Machinery gene
X875_RS06380 (X875_12650)	radA	1352094..1353473 (+)	1380	WP_025247570.1	DNA repair protein RadA	-
X875_RS06385 (X875_12660)	-	1353527..1353760 (-)	234	WP_025247569.1	YgjV family protein	-

Sequence

Protein

• Length: 398 a.a.
• Molecular weight: 45543.01 Da
• Isoelectric Point: 9.3236

>NTDB_id=115172 X875_RS06365 WP_025342724.1 1348834..1350030(-) (pilC) [Mannheimia varigena USDA-ARS-USMARC-1388]
MAKIYEFHWKAVDRFQQKRKGRLLSSSREELESTLLAKGYEQIRVSRNFVFSQNPKKEEISQFLSQLALLVNSAVPLKQA
LFLILQNCRNIRLYQWLSELIQLIESGYFFSQSLEKLGKFIANQEIQLIKMGEQSGKLGLILTNLAESRLKSDKLAKKVK
KILFYPAIVLFISFSLSIGLLVFIVPQFAELYGTKEQALPLITEILFSLSQCLIEQKNLLFSLFLLAVIFFLFFAKRGNW
AAKLKISVLSKLPVFRQIIEQARIVFFSQNIALMLNAHIPLDVALKSFLSEKSDDPILQKEVEFMLNLLQQGYKFSQGIN
SNVFGLDMLQMIEIGEQSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLLMGVIVGTIIVGLYLPIFDIGSLVE

Nucleotide

• Length: 1197 bp

>NTDB_id=115172 X875_RS06365 WP_025342724.1 1348834..1350030(-) (pilC) [Mannheimia varigena USDA-ARS-USMARC-1388]
ATGGCTAAAATTTATGAATTTCATTGGAAAGCGGTGGATCGTTTCCAACAAAAGCGTAAAGGCAGGCTATTATCTTCAAG
CCGAGAAGAGCTTGAAAGCACATTACTTGCAAAAGGTTATGAACAAATTCGAGTTAGCCGAAATTTTGTCTTTTCGCAAA
ATCCGAAAAAAGAGGAGATCAGCCAATTTTTATCTCAACTGGCATTGTTAGTGAATTCTGCCGTTCCATTAAAGCAAGCT
CTCTTTTTGATTTTACAAAATTGCCGAAATATTAGGTTATATCAATGGCTAAGTGAATTAATTCAGTTAATTGAAAGCGG
CTATTTTTTTTCGCAATCACTGGAAAAATTAGGGAAATTTATCGCTAACCAAGAAATTCAACTAATTAAAATGGGCGAGC
AGAGCGGAAAACTTGGTTTGATTTTAACCAACTTGGCGGAGAGCAGACTTAAATCGGATAAACTGGCTAAAAAAGTCAAG
AAAATCCTGTTTTATCCAGCAATTGTACTGTTTATCTCATTCTCTCTTTCTATCGGACTTTTGGTATTCATCGTGCCACA
ATTTGCCGAACTTTACGGCACAAAAGAACAAGCCTTACCGCTGATTACTGAGATTTTATTTTCTCTTTCTCAATGTTTGA
TTGAACAGAAGAATCTTTTATTCAGTTTATTTTTACTAGCGGTTATTTTTTTCTTATTTTTTGCAAAAAGAGGGAATTGG
GCAGCGAAATTGAAAATCAGCGTATTGTCTAAGCTCCCTGTTTTTAGACAAATTATAGAGCAAGCTCGCATTGTCTTTTT
TAGCCAAAATATTGCGTTGATGTTGAATGCTCATATTCCATTAGATGTCGCATTAAAATCCTTTTTATCTGAAAAAAGTG
ATGACCCTATATTACAAAAAGAAGTTGAGTTTATGCTCAATCTCTTACAACAAGGCTATAAGTTTTCGCAAGGTATCAAC
TCAAATGTGTTTGGCTTAGATATGCTTCAGATGATTGAGATAGGCGAACAGAGCGGTAATGTTGCTAAAATGTGTGAGCA
TATTAGCGAGATGTATCAACAAAAACTGGATTATCAAATTGATATTCTCTCCCAATTATTAGAGCCAATGCTGATGCTAT
TAATGGGCGTAATTGTCGGCACTATTATTGTTGGGCTGTACTTGCCTATTTTCGATATAGGCTCATTGGTGGAGTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (267-389)

• Type II secretion system (T2SS), protein F (T2SSF) (63-186)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Glaesserella parasuis strain SC1401	55.86	100	0.563
pilC	Haemophilus influenzae Rd KW20	42.394	100	0.427
pilC	Haemophilus influenzae 86-028NP	41.397	100	0.417