Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KOD61_RS02995 Genome accession   NZ_OU015430
Coordinates   644545..645603 (+) Length   352 a.a.
NCBI ID   WP_215219587.1    Uniprot ID   -
Organism   Novilysobacter luteus strain CECT 30171     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 639545..650603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOD61_RS02980 (LYB30171_00600) - 639686..640984 (+) 1299 WP_215219585.1 citrate synthase -
  KOD61_RS02985 (LYB30171_00601) - 641056..641697 (-) 642 WP_215220257.1 hypothetical protein -
  KOD61_RS02990 (LYB30171_00602) - 641799..644327 (-) 2529 WP_215219586.1 penicillin-binding protein 1A -
  KOD61_RS02995 (LYB30171_00603) pilM 644545..645603 (+) 1059 WP_215219587.1 pilus assembly protein PilM Machinery gene
  KOD61_RS03000 (LYB30171_00604) - 645603..646361 (+) 759 WP_215219588.1 PilN domain-containing protein -
  KOD61_RS03005 (LYB30171_00605) - 646358..647017 (+) 660 WP_215219589.1 type 4a pilus biogenesis protein PilO -
  KOD61_RS03010 (LYB30171_00606) - 647052..647573 (+) 522 WP_251370682.1 pilus assembly protein PilP -
  KOD61_RS03015 (LYB30171_00607) - 647593..649533 (+) 1941 WP_215219591.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37474.90 Da        Isoelectric Point: 4.4671

>NTDB_id=1150989 KOD61_RS02995 WP_215219587.1 644545..645603(+) (pilM) [Novilysobacter luteus strain CECT 30171]
MGVITKSQPALVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEKNIVEPEAVGEAIKRAVARSGTRAKYAAAA
VAGSAVITKVIPMSADLEGDDLEAQVELEAVNYVPYPIEEVNLDFEVLGPVPGSNESVQVLLAASRAENVEVRASALEIG
GLTPKVMDVEAFAIENAFALLADQLSAPKDGLVALIDSGATMTTLNVIRNGRSLYSREQVFGGKQLTDEVMRRYSLSYEE
AGLAKRQGGLPDTYETEVLDPFKEAMVQQISRLLQFFYAGSEYNRVDQIVVAGGCAAIPGIAGMIEEQLGVPTMIANPLA
HMTLGPRVQAHALAQDAPALLIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1150989 KOD61_RS02995 WP_215219587.1 644545..645603(+) (pilM) [Novilysobacter luteus strain CECT 30171]
GTGGGTGTCATTACCAAGAGCCAGCCGGCGCTCGTCGGCGTGGATATCAGTTCGACTGCGGTCAAGCTTCTGCAGCTGTC
GCGGGCGGGCAACCGCTACCGCGTCGAGCACTACGCGGTGGAGCCGTTGCCGCCCAACGCGGTGGTCGAGAAGAACATCG
TCGAGCCCGAAGCGGTCGGCGAAGCGATCAAGCGCGCGGTCGCGCGGTCCGGTACCCGGGCCAAGTACGCCGCCGCGGCG
GTGGCCGGTTCGGCGGTGATCACGAAGGTGATCCCGATGTCGGCCGACCTGGAAGGCGACGACCTGGAAGCGCAGGTCGA
GCTGGAAGCGGTCAACTACGTGCCGTACCCGATCGAGGAAGTGAACCTCGACTTCGAGGTGCTGGGCCCGGTGCCCGGCA
GCAACGAGTCGGTGCAGGTCCTGCTGGCCGCCTCGCGCGCCGAGAACGTCGAGGTCCGCGCGTCGGCGCTGGAGATCGGC
GGTTTGACGCCGAAGGTGATGGACGTGGAGGCGTTCGCGATCGAGAACGCGTTCGCGCTCCTCGCCGACCAGCTCAGCGC
GCCGAAGGACGGCCTGGTCGCCCTGATCGATTCCGGGGCGACGATGACCACCCTCAACGTCATCCGCAACGGCCGCAGCC
TCTACAGCCGCGAGCAGGTGTTTGGCGGCAAGCAGCTGACCGACGAGGTCATGCGCCGCTACAGCCTCAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGCGGGCTGCCGGATACCTACGAAACCGAGGTGCTGGATCCGTTCAAGGAGGCGATGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCCGGCAGCGAGTACAACCGCGTCGACCAGATCGTCGTCGCCGGCG
GCTGCGCGGCCATCCCGGGCATCGCCGGGATGATCGAGGAGCAGCTGGGCGTCCCGACCATGATCGCCAACCCGCTGGCC
CACATGACCCTTGGTCCGCGCGTGCAGGCGCATGCCCTCGCCCAGGACGCGCCCGCCCTGCTGATCGCCTGCGGCCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.009

98.58

0.503
  comM Acinetobacter nosocomialis M2

51.009

98.58

0.503
  comM Acinetobacter baylyi ADP1

49.568

98.58

0.489
  pilM Legionella pneumophila strain ERS1305867

45.058

97.727

0.44

Multiple sequence alignment