Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   X808_RS03115 Genome accession   NZ_CP006943
Coordinates   652315..653628 (-) Length   437 a.a.
NCBI ID   WP_025216881.1    Uniprot ID   -
Organism   Mannheimia varigena USDA-ARS-USMARC-1296     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 647315..658628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  X808_RS03085 (X808_6040) ruvB 647943..648947 (-) 1005 WP_025216875.1 Holliday junction branch migration DNA helicase RuvB -
  X808_RS03090 (X808_6050) ruvA 648958..649563 (-) 606 WP_025216876.1 Holliday junction branch migration protein RuvA -
  X808_RS03095 (X808_6060) - 649672..650298 (-) 627 WP_025216877.1 LysE family transporter -
  X808_RS03100 (X808_6070) - 650300..650773 (-) 474 WP_025216878.1 phosphatidylglycerophosphatase A -
  X808_RS03105 (X808_6080) thiL 650779..651759 (-) 981 WP_025216879.1 thiamine-phosphate kinase -
  X808_RS03110 (X808_6090) nusB 651822..652235 (-) 414 WP_025216880.1 transcription antitermination factor NusB -
  X808_RS03115 (X808_6100) comE 652315..653628 (-) 1314 WP_025216881.1 type IV pilus secretin PilQ Machinery gene
  X808_RS03120 (X808_6110) - 653637..654050 (-) 414 WP_025216882.1 hypothetical protein -
  X808_RS03125 (X808_6120) - 654052..654585 (-) 534 WP_025216883.1 hypothetical protein -
  X808_RS03130 (X808_6130) - 654587..655108 (-) 522 WP_025216884.1 hypothetical protein -
  X808_RS03135 (X808_6140) - 655084..655785 (-) 702 WP_025216885.1 hypothetical protein -
  X808_RS03140 (X808_6150) - 655911..658463 (+) 2553 WP_025216886.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48363.39 Da        Isoelectric Point: 8.9446

>NTDB_id=115047 X808_RS03115 WP_025216881.1 652315..653628(-) (comE) [Mannheimia varigena USDA-ARS-USMARC-1296]
MFRIFLYLLLSSSLALADTISLAVRNAPTSLLLTYLAEETGKNIVLDDDIQTKSTLRLENKSINEIFKTLSKVNKLSFTQ
EADIVYISKKEEKSADLNSVSIAHLQNNIQNPPLVTSPKLITKTIKLNYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSAKSLQNIERLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSRGTDHYKFGGRLEGNGLSNVANNLNVN
FPVANGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNTQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKVRGVKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=115047 X808_RS03115 WP_025216881.1 652315..653628(-) (comE) [Mannheimia varigena USDA-ARS-USMARC-1296]
ATGTTTAGAATTTTTCTGTATTTATTACTTAGTTCGTCATTAGCATTGGCTGACACTATTTCCCTTGCAGTAAGGAATGC
ACCAACTTCACTTCTGCTAACCTATTTAGCTGAAGAAACAGGTAAAAATATAGTATTAGATGATGATATTCAAACTAAAT
CCACCTTAAGATTAGAAAATAAATCCATTAATGAGATTTTTAAAACATTGAGCAAAGTAAATAAATTATCTTTTACTCAA
GAAGCAGACATTGTCTATATTAGTAAAAAAGAAGAAAAATCGGCTGATTTAAATTCAGTTTCAATTGCCCATTTGCAAAA
TAATATACAAAACCCACCGCTTGTAACTTCTCCAAAACTGATAACTAAAACTATAAAACTAAATTATGCTAAAGCCTCAG
AAGTGATTGAATCTTTAACTAAAGGCAGTGGCACGTTTTTATCTGAAAATGGTTATATCCATTTTGACGAGCGAAGTAAT
AGCTTAATTATCAAAGATAGTGCGAAATCACTACAAAATATTGAGCGATTAGTCAAACAACTCGACCAACCCACCGAACA
AATTGCGATTGAAGCTCGCATTGTCACGATTAGTAGCGAACATTTGCAAGAATTAGGTGTGCGTTGGGGAATGTTTTCCA
GAGGAACTGATCACTATAAATTTGGTGGGCGATTGGAAGGAAATGGGTTAAGCAATGTAGCTAATAATCTCAATGTGAAT
TTCCCCGTTGCGAATGGTGCTTCAGCAGTTTTACAAGTAGCTTCCATTAATAGTCGAGTACTAGATTTAGAACTCAGTGC
ATTAGAACAGGAAAACAGTGTGGAAATCATTGCTAGCCCTCGTCTTTTAACCACTAATAAAAAACCTGCGAGTATCAAGC
AAGGCACGGAAATTCCTTATGTAATGTATAACACCAAATCAGAAGCAACCGATGTCGAATTTAAAGAAGCAGTGTTAGGA
TTGGAAGTTGTTCCTCATCTTTCTACTCAAAATCAAATTTTGTTGGATTTAATCGTCACTCAAAACTCACCGAATACACA
ATCTGGTAGCAGTGGTTTAATTACCATTGATAAGCAAGAACTTAATACACAGGTGTTTGCTAAACACGGTGAAACTATCG
TGCTAGGTGGTATTTTCCAACATTTAACCCAAAAAGGAGAAGATAAAGTACCTATTTTAGGCTCAATTCCGTTTATTAAA
CGATTATTTAGCCAAACAAGAGACAAAATCAGTAAGCGGGAACTTGTTATTTTTGTTACCCCTTACATTCTCCAATCAAA
CGAAAAAGTAAGGGGCGTGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.448

99.542

0.632
  comE Haemophilus influenzae 86-028NP

48.115

100

0.497
  comE Haemophilus influenzae Rd KW20

51.09

94.508

0.483
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.094

97.025

0.389
  pilQ Vibrio cholerae strain A1552

40.094

97.025

0.389
  pilQ Vibrio campbellii strain DS40M4

38.283

98.627

0.378

Multiple sequence alignment