Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   X781_RS08270 Genome accession   NZ_CP006942
Coordinates   1725842..1727155 (+) Length   437 a.a.
NCBI ID   WP_025236416.1    Uniprot ID   -
Organism   Mannheimia sp. USDA-ARS-USMARC-1261     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1720842..1732155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  X781_RS08245 (X781_16840) - 1721013..1723559 (-) 2547 WP_025236411.1 penicillin-binding protein 1A -
  X781_RS08250 (X781_16850) - 1723685..1724386 (+) 702 WP_025236412.1 hypothetical protein -
  X781_RS08255 (X781_16860) - 1724362..1724883 (+) 522 WP_025236413.1 hypothetical protein -
  X781_RS08260 (X781_16870) - 1724885..1725418 (+) 534 WP_025236414.1 hypothetical protein -
  X781_RS08265 (X781_16880) - 1725420..1725833 (+) 414 WP_025236415.1 hypothetical protein -
  X781_RS08270 (X781_16890) comE 1725842..1727155 (+) 1314 WP_025236416.1 type IV pilus secretin PilQ Machinery gene
  X781_RS08275 (X781_16900) nusB 1727235..1727648 (+) 414 WP_025236417.1 transcription antitermination factor NusB -
  X781_RS08280 (X781_16910) thiL 1727711..1728691 (+) 981 WP_025236418.1 thiamine-phosphate kinase -
  X781_RS08285 (X781_16920) - 1728697..1729170 (+) 474 WP_025236419.1 phosphatidylglycerophosphatase A -
  X781_RS08290 (X781_16930) - 1729176..1729802 (+) 627 WP_025236420.1 LysE family transporter -
  X781_RS08295 (X781_16940) ruvA 1729911..1730516 (+) 606 WP_025236421.1 Holliday junction branch migration protein RuvA -
  X781_RS08300 (X781_16950) ruvB 1730527..1731531 (+) 1005 WP_025236422.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48427.44 Da        Isoelectric Point: 8.2948

>NTDB_id=115025 X781_RS08270 WP_025236416.1 1725842..1727155(+) (comE) [Mannheimia sp. USDA-ARS-USMARC-1261]
MFRVFLYLLFSSSLALADTISLAVKNVPTSHLLTYLSEETGKNIVLEDDIQTKSTLRLENKSIEEIFKTLSKVNKLSLTY
EGDIVYINKKEEKIADLNTIPITHLQNNGQNPPLVTTPKLMTKTIKLNYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSAKSLQNIERLVKKLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSRGADHYKFGGRLEGNGLSNVANNLNVN
FPVTNGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLVVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLAQKGEDKVPVLGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKVSSTKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=115025 X781_RS08270 WP_025236416.1 1725842..1727155(+) (comE) [Mannheimia sp. USDA-ARS-USMARC-1261]
ATGTTCAGAGTTTTTCTTTATTTATTATTTAGCTCTTCACTAGCGTTGGCTGATACTATTTCTCTTGCAGTAAAAAATGT
ACCAACTTCACATCTGCTTACTTATTTATCCGAAGAAACAGGGAAAAATATAGTATTAGAGGATGATATTCAAACTAAAT
CTACATTAAGATTAGAAAACAAATCTATTGAAGAGATATTTAAAACACTCAGCAAAGTGAATAAATTATCGCTCACTTAT
GAAGGTGATATTGTTTATATTAATAAGAAAGAAGAAAAAATAGCTGATTTGAATACAATACCTATTACCCATTTGCAAAA
TAACGGACAAAATCCACCGCTTGTAACCACTCCAAAGTTGATGACTAAAACGATAAAGCTCAATTATGCGAAAGCCTCAG
AAGTGATTGAATCTTTAACTAAAGGTAGTGGTACGTTTTTATCTGAGAATGGCTATATCCATTTTGATGAGCGAAGTAAT
AGCTTAATTATTAAAGATAGTGCGAAATCACTGCAAAATATTGAGCGATTAGTCAAAAAACTAGACCAACCTACCGAACA
AATTGCGATTGAAGCTCGCATTGTTACTATCAGTAGCGAACATTTGCAAGAACTAGGAGTGCGTTGGGGAATGTTTTCCA
GAGGAGCTGATCACTATAAATTTGGCGGGCGACTTGAAGGTAATGGATTAAGTAATGTAGCTAATAATCTCAATGTGAAT
TTCCCTGTTACGAATGGAGCTTCCGCCGTTTTACAAGTGGCTTCCATTAATAGCCGTGTGCTAGATTTGGAACTTAGTGC
ATTAGAGCAGGAAAACAGTGTGGAAATTATTGCCAGTCCTCGTCTTTTAACCACGAATAAAAAACCTGCAAGCATCAAGC
AAGGTACAGAAATTCCTTATGTAATGTATAACACCAAATCAGAAGCAACCGATGTCGAATTTAAAGAAGCAGTGTTAGGA
TTGGAAGTTGTTCCTCATCTTTCTACTCAAAATCAAATTTTGCTGGATTTAGTCGTAACCCAAAACTCGCCGAACTCTCA
ATCCGGCAGTAGCGGTTTAATTACTATTGATAAGCAAGAACTCAACACACAAGTTTTTGCTAAACACGGGGAAACTATTG
TATTAGGTGGAATTTTTCAACATTTAGCCCAAAAAGGGGAAGATAAAGTGCCTGTTTTAGGTTCAATTCCCTTTATTAAG
CGTTTATTCAGCCAAACTAGAGATAAAATCAGCAAACGAGAACTCGTCATTTTCGTTACTCCTTACATTCTTCAATCAAA
CGAAAAAGTAAGCAGTACGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

62.903

99.314

0.625
  comE Haemophilus influenzae Rd KW20

51.932

94.737

0.492
  comE Haemophilus influenzae 86-028NP

49.078

99.314

0.487
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.573

96.568

0.382
  pilQ Vibrio cholerae strain A1552

39.573

96.568

0.382
  pilQ Vibrio campbellii strain DS40M4

38.084

97.941

0.373

Multiple sequence alignment