Detailed information NTG1148965

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DTQ61_RS16500
• Genome accession: NZ_LT985192
• Coordinates: 3435159..3436328 (-)
• Length: 389 a.a.
• NCBI ID: WP_044324086.1
• Uniprot ID: A0AB35R597
• Organism: Pseudomonas syringae strain CFBP 2116
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3430159..3441328

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DTQ61_RS16485 (PSCFBP2116_03167)	-	3431443..3432507 (+)	1065	WP_003364338.1	microcin C ABC transporter permease YejB	-
DTQ61_RS16490 (PSCFBP2116_03168)	-	3432507..3433529 (+)	1023	WP_003435512.1	ABC transporter permease	-
DTQ61_RS16495 (PSCFBP2116_03169)	-	3433531..3435108 (+)	1578	WP_060407418.1	ABC transporter ATP-binding protein	-
DTQ61_RS16500 (PSCFBP2116_03170)	pilU	3435159..3436328 (-)	1170	WP_044324086.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DTQ61_RS16505 (PSCFBP2116_03171)	-	3436452..3436727 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
DTQ61_RS16510 (PSCFBP2116_03172)	pgm	3436802..3438448 (-)	1647	WP_044324087.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
DTQ61_RS16515 (PSCFBP2116_03173)	-	3438564..3440891 (-)	2328	WP_060404437.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42645.81 Da
• Isoelectric Point: 6.0641

>NTDB_id=1148965 DTQ61_RS16500 WP_044324086.1 3435159..3436328(-) (pilU) [Pseudomonas syringae strain CFBP 2116]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPIGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1148965 DTQ61_RS16500 WP_044324086.1 3435159..3436328(-) (pilU) [Pseudomonas syringae strain CFBP 2116]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTACCTTTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAATGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGAGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAGTTTCAGCGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCCGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAAGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTCGATACCCGAAGCTTCCGCGC
GGCGCTGAAGAACACGTTACGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAAGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGATCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.261	91.774	0.47
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386