Detailed information NTG1148868

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C6H39_RS12415
• Genome accession: NZ_LT963409
• Coordinates: 2589940..2591109 (+)
• Length: 389 a.a.
• NCBI ID: WP_044324086.1
• Uniprot ID: A0AB35R597
• Organism: Pseudomonas syringae isolate CFBP3840
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2584940..2596109

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H39_RS12400 (CFBP3840_02383)	-	2585377..2587704 (+)	2328	WP_060404437.1	ATP-dependent DNA helicase	-
C6H39_RS12405 (CFBP3840_02384)	pgm	2587820..2589466 (+)	1647	WP_044324087.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
C6H39_RS12410 (CFBP3840_02385)	-	2589541..2589816 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
C6H39_RS12415 (CFBP3840_02386)	pilU	2589940..2591109 (+)	1170	WP_044324086.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C6H39_RS12420 (CFBP3840_02387)	-	2591160..2592737 (-)	1578	WP_060407418.1	ABC transporter ATP-binding protein	-
C6H39_RS12425 (CFBP3840_02388)	-	2592739..2593761 (-)	1023	WP_003435512.1	ABC transporter permease	-
C6H39_RS12430 (CFBP3840_02389)	-	2593761..2594825 (-)	1065	WP_003364338.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42645.81 Da
• Isoelectric Point: 6.0641

>NTDB_id=1148868 C6H39_RS12415 WP_044324086.1 2589940..2591109(+) (pilU) [Pseudomonas syringae isolate CFBP3840]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPIGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1148868 C6H39_RS12415 WP_044324086.1 2589940..2591109(+) (pilU) [Pseudomonas syringae isolate CFBP3840]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTACCTTTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAATGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGAGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAGTTTCAGCGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCCGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAAGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTCGATACCCGAAGCTTCCGCGC
GGCGCTGAAGAACACGTTACGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAAGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGATCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.261	91.774	0.47
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386