Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   CKV85_RS08955 Genome accession   NZ_LT906454
Coordinates   1836983..1838281 (-) Length   432 a.a.
NCBI ID   WP_095123387.1    Uniprot ID   -
Organism   Streptococcus acidominimus strain NCTC11291     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1831983..1843281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV85_RS08945 (SAMEA4504048_01911) hpf 1835691..1836239 (-) 549 WP_017769993.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CKV85_RS08950 (SAMEA4504048_01912) comFC/cflB 1836321..1836983 (-) 663 WP_095123385.1 ComF family protein Machinery gene
  CKV85_RS08955 (SAMEA4504048_01913) comFA/cflA 1836983..1838281 (-) 1299 WP_095123387.1 DEAD/DEAH box helicase Machinery gene
  CKV85_RS08960 (SAMEA4504048_01914) - 1838329..1838955 (+) 627 WP_095123389.1 YigZ family protein -
  CKV85_RS08965 (SAMEA4504048_01915) cysK 1839051..1839971 (+) 921 WP_095123391.1 cysteine synthase A -
  CKV85_RS08970 (SAMEA4504048_01916) - 1840246..1841109 (+) 864 WP_095122103.1 IS982 family transposase -
  CKV85_RS08975 (SAMEA4504048_01917) - 1841403..1842208 (-) 806 Protein_1774 formate/nitrite transporter family protein -
  CKV85_RS08980 (SAMEA4504048_01919) - 1842661..1843023 (-) 363 WP_029691591.1 S1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49505.37 Da        Isoelectric Point: 9.3453

>NTDB_id=1147500 CKV85_RS08955 WP_095123387.1 1836983..1838281(-) (comFA/cflA) [Streptococcus acidominimus strain NCTC11291]
MKKSQEKAGCWFTQEDLLPEDLQMAVPLDALIKQQDTWYCRRCGVSIRQNWKLPTGYYYCRSCILLGRLTQENHLYYFPP
KSFPHSDCLKWGGQLTPYQQNISDKLVKAIEDRQDSLVHAVTGAGKTEMIYQAVATTINQGGQVCLASPRIDVCLELYNR
LVRDFSCDVDLLHGQSQPYSGAPLVVATTHQMLKFYKCFDLILVDEVDAFPYVDDPVLYHAVSTAKKDSGVTIFMTATST
KALDKRVRRKELQKLTLSRRFHGNPLIIPKTLWLSSLEKQVHEGRLPKKLERQLIKQLSTDYPLLIFVPEIDFGKTLTNI
FHRYFPQVTTKFVASTSLDRVELVQQFREGTTRILITTTILERGVTFPKVDVFVLLAHHRLFTSSSLIQISGRVGRSMER
PTGDLFFFHEGLTKAIKETRREIKKMNQLGGF

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1147500 CKV85_RS08955 WP_095123387.1 1836983..1838281(-) (comFA/cflA) [Streptococcus acidominimus strain NCTC11291]
ATGAAAAAAAGTCAAGAAAAAGCTGGGTGCTGGTTTACTCAAGAGGATTTATTACCCGAAGATTTACAGATGGCAGTTCC
TCTAGATGCTCTTATCAAACAACAAGATACTTGGTATTGTCGCCGATGTGGAGTATCTATTCGTCAAAATTGGAAACTTC
CAACAGGATACTATTATTGTAGGTCTTGTATTCTTTTGGGACGTTTAACCCAAGAGAATCACTTATATTATTTTCCACCC
AAGTCATTTCCTCATAGTGATTGTTTAAAATGGGGCGGTCAACTCACGCCTTATCAGCAAAACATTTCAGATAAGCTTGT
TAAAGCTATCGAAGATAGACAAGATAGTTTAGTTCATGCTGTGACAGGTGCAGGTAAGACAGAAATGATTTATCAAGCTG
TTGCTACAACAATCAATCAAGGTGGGCAGGTCTGTTTGGCTAGTCCTAGAATTGATGTCTGTTTGGAATTGTACAACAGG
TTGGTGCGTGACTTCTCTTGTGATGTTGACTTACTACATGGCCAATCACAACCTTACAGTGGCGCGCCTTTGGTCGTTGC
CACCACTCATCAGATGCTAAAATTTTATAAGTGTTTTGATTTAATTCTGGTCGATGAAGTGGATGCTTTTCCTTATGTCG
ATGATCCTGTACTTTATCATGCCGTTTCAACTGCCAAAAAGGATAGTGGTGTTACCATTTTCATGACTGCGACCTCAACC
AAGGCATTAGATAAACGAGTTAGACGCAAAGAATTACAAAAATTAACCTTGTCACGCCGATTCCACGGTAATCCTTTGAT
CATTCCAAAAACACTTTGGCTTTCTTCTTTGGAGAAGCAAGTACATGAAGGACGTCTGCCAAAGAAACTAGAGCGCCAAT
TAATAAAGCAGCTATCCACAGATTATCCTTTACTGATTTTTGTCCCTGAAATTGACTTTGGAAAAACATTAACCAATATA
TTTCATCGTTATTTTCCACAAGTAACCACTAAATTTGTGGCAAGTACCAGTTTAGATAGGGTTGAACTAGTTCAGCAGTT
TAGAGAAGGGACGACTCGTATTTTAATAACAACTACAATCTTGGAAAGAGGAGTTACTTTTCCAAAGGTAGATGTTTTTG
TACTTCTAGCACACCATAGGTTGTTTACTAGTTCCAGTCTGATTCAGATTTCAGGCCGCGTCGGAAGATCCATGGAGCGC
CCGACAGGAGATCTGTTCTTTTTTCATGAAGGATTGACTAAAGCTATCAAAGAAACTAGGAGGGAAATCAAAAAAATGAA
CCAGTTAGGAGGATTTTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-163)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

57.87

100

0.579
  comFA/cflA Streptococcus pneumoniae D39

57.87

100

0.579
  comFA/cflA Streptococcus pneumoniae R6

57.87

100

0.579
  comFA/cflA Streptococcus pneumoniae TIGR4

57.639

100

0.576
  comFA/cflA Streptococcus mitis NCTC 12261

57.176

100

0.572
  comFA/cflA Streptococcus mitis SK321

56.481

100

0.565
  comFA Lactococcus lactis subsp. cremoris KW2

48.477

91.204

0.442
  comFA Latilactobacillus sakei subsp. sakei 23K

39.012

93.75

0.366

Multiple sequence alignment