Detailed information NTG1145605

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BT137_RS04245
• Genome accession: NZ_LT632617
• Coordinates: 899145..900209 (+)
• Length: 354 a.a.
• NCBI ID: WP_014843599.1
• Uniprot ID: A0A4T1VG56
• Organism: Legionella pneumophila strain ST42 isolate ST42
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 894145..905209

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BT137_RS04235 (EUL120_00843)	-	895185..897569 (-)	2385	WP_014843597.1	penicillin-binding protein 1A	-
BT137_RS04240 (EUL120_00844)	-	897721..898728 (-)	1008	WP_014843598.1	hypothetical protein	-
BT137_RS04245 (EUL120_00845)	pilM	899145..900209 (+)	1065	WP_014843599.1	type IV pilus assembly protein PilM	Machinery gene
BT137_RS04250 (EUL120_00846)	pilN	900213..900761 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
BT137_RS04255 (EUL120_00847)	pilO	900775..901371 (+)	597	WP_027224065.1	type 4a pilus biogenesis protein PilO	Machinery gene
BT137_RS04260 (EUL120_00848)	pilP	901368..901952 (+)	585	WP_014843601.1	pilus assembly protein PilP	Machinery gene
BT137_RS04265 (EUL120_00849)	pilQ	901956..904052 (+)	2097	WP_014843602.1	type IV pilus secretin PilQ	Machinery gene
BT137_RS04270 (EUL120_00850)	aroK	904497..905024 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38776.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=1145605 BT137_RS04245 WP_014843599.1 899145..900209(+) (pilM) [Legionella pneumophila strain ST42 isolate ST42]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLASLIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1145605 BT137_RS04245 WP_014843599.1 899145..900209(+) (pilM) [Legionella pneumophila strain ST42 isolate ST42]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCGGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGCCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCATCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A4T1VG56

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407