Detailed information NTG1145482

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BT141_RS04990
• Genome accession: NZ_LT632614
• Coordinates: 1076544..1077608 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain ST62 isolate ST62
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1071544..1082608

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BT141_RS04980 (H044120014_00973)	-	1072584..1074968 (-)	2385	WP_027220940.1	penicillin-binding protein 1A	-
BT141_RS04985 (H044120014_00974)	ravI	1075120..1076127 (-)	1008	WP_011946020.1	Dot/Icm T4SS effector RavI	-
BT141_RS04990 (H044120014_00975)	pilM	1076544..1077608 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
BT141_RS04995 (H044120014_00976)	pilN	1077612..1078160 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
BT141_RS05000 (H044120014_00977)	pilO	1078174..1078773 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
BT141_RS05005 (H044120014_00978)	pilP	1078770..1079354 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
BT141_RS05010 (H044120014_00979)	pilQ	1079358..1081454 (+)	2097	WP_027220941.1	type IV pilus secretin PilQ	Machinery gene
BT141_RS05015 (H044120014_00980)	aroK	1081900..1082427 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=1145482 BT141_RS04990 WP_011215094.1 1076544..1077608(+) (pilM) [Legionella pneumophila strain ST62 isolate ST62]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1145482 BT141_RS04990 WP_011215094.1 1076544..1077608(+) (pilM) [Legionella pneumophila strain ST62 isolate ST62]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407