Detailed information NTG1145243

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PHAGEPCYII10_RS02100
• Genome accession: NZ_LT608330
• Coordinates: 452518..453666 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa isolate PA14Or_reads
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 447518..458666

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PHAGEPCYII10_RS02075	-	448669..448965 (-)	297	WP_031632852.1	DUF167 domain-containing protein	-
PHAGEPCYII10_RS02080 (PA14OR_0411)	-	448962..449555 (-)	594	WP_003084544.1	YggT family protein	-
PHAGEPCYII10_RS02085	proC	449566..450387 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PHAGEPCYII10_RS02090 (PA14OR_0413)	-	450399..451091 (-)	693	WP_004365038.1	YggS family pyridoxal phosphate-dependent enzyme	-
PHAGEPCYII10_RS02095	pilT	451306..452340 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PHAGEPCYII10_RS02100	pilU	452518..453666 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PHAGEPCYII10_RS02105	czcD	453674..454573 (-)	900	WP_004365039.1	CDF family zinc transporter CzcD	-
PHAGEPCYII10_RS02110 (PA14OR_0417)	-	454728..455132 (+)	405	WP_003084560.1	NINE protein	-
PHAGEPCYII10_RS02115 (PA14OR_0418)	-	455375..456748 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
PHAGEPCYII10_RS02120 (PA14OR_0419)	-	456745..457929 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1145243 PHAGEPCYII10_RS02100 WP_003110016.1 452518..453666(+) (pilU) [Pseudomonas aeruginosa isolate PA14Or_reads]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1145243 PHAGEPCYII10_RS02100 WP_003110016.1 452518..453666(+) (pilU) [Pseudomonas aeruginosa isolate PA14Or_reads]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTATATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382