Detailed information NTG1143662

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PCPL58_RS26430
• Genome accession: NZ_LT222319
• Coordinates: 5405657..5406721 (-)
• Length: 354 a.a.
• NCBI ID: WP_003365992.1
• Uniprot ID: A0A193SY06
• Organism: Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5400657..5411721

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCPL58_RS26405 (PCPL58_4826)	aroK	5401262..5401780 (-)	519	WP_003365999.1	shikimate kinase AroK	-
PCPL58_RS26410 (PCPL58_4827)	pilQ	5401785..5403890 (-)	2106	WP_003432146.1	type IV pilus secretin PilQ	Machinery gene
PCPL58_RS26415 (PCPL58_4828)	pilP	5403938..5404465 (-)	528	WP_003350151.1	type 4a pilus biogenesis lipoprotein PilP	-
PCPL58_RS26420 (PCPL58_4829)	pilO	5404462..5405085 (-)	624	WP_003432147.1	type 4a pilus biogenesis protein PilO	-
PCPL58_RS26425 (PCPL58_4830)	-	5405082..5405657 (-)	576	WP_003365994.1	PilN domain-containing protein	-
PCPL58_RS26430 (PCPL58_4831)	pilM	5405657..5406721 (-)	1065	WP_003365992.1	pilus assembly protein PilM	Machinery gene
PCPL58_RS26435 (PCPL58_4832)	-	5406931..5409372 (+)	2442	WP_414860623.1	penicillin-binding protein 1A	-
PCPL58_RS26440 (PCPL58_4833)	-	5410100..5411368 (-)	1269	WP_003365988.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38104.65 Da
• Isoelectric Point: 4.7049

>NTDB_id=1143662 PCPL58_RS26430 WP_003365992.1 5405657..5406721(-) (pilM) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1143662 PCPL58_RS26430 WP_003365992.1 5405657..5406721(-) (pilM) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCCTTGCCGGCCAACGCCGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTACTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAGGCTGATCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAGAACGTTGAAGTGCGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCGCGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGCTCTTTCGGGCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCACGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGACTACGTCAGCGAAGTGCTCGACCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGCTCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCTGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGACGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCGCCAAGGTCAATGCCGGGGCCCTGGCCAGTGACGCGCCAGCCCTGATGATCGCCTGTGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A193SY06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.932	100	0.559
comM	Acinetobacter nosocomialis M2	55.932	100	0.559
comM	Acinetobacter baylyi ADP1	53.955	100	0.54
pilM	Legionella pneumophila strain ERS1305867	46.479	100	0.466