Detailed information NTG1143368

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PA5486_RS02460
• Genome accession: NZ_LS998783
• Coordinates: 494778..495926 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa isolate
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 489778..500926

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA5486_RS02435	-	490929..491225 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PA5486_RS02440 (PA5486_00457)	-	491222..491815 (-)	594	WP_003084544.1	YggT family protein	-
PA5486_RS02445 (PA5486_00458)	proC	491826..492647 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PA5486_RS02450 (PA5486_00459)	-	492659..493351 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
PA5486_RS02455 (PA5486_00460)	pilT	493566..494600 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PA5486_RS02460 (PA5486_00461)	pilU	494778..495926 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PA5486_RS02465 (PA5486_00462)	czcD	495934..496833 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PA5486_RS02470 (PA5486_00463)	-	496987..497391 (+)	405	WP_003084560.1	NINE protein	-
PA5486_RS02475 (PA5486_00464)	-	497634..499007 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PA5486_RS02480 (PA5486_00465)	-	499004..500188 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1143368 PA5486_RS02460 WP_003100959.1 494778..495926(+) (pilU) [Pseudomonas aeruginosa isolate]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1143368 PA5486_RS02460 WP_003100959.1 494778..495926(+) (pilU) [Pseudomonas aeruginosa isolate]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382