Detailed information NTG1142382

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DQM71_RS14860
• Genome accession: NZ_LS483472
• Coordinates: 3059352..3060470 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain NCTC13421
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3054352..3065470

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM71_RS14850 (NCTC13421_02968)	-	3057468..3058160 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
DQM71_RS14855 (NCTC13421_02969)	pilT	3058287..3059324 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
DQM71_RS14860 (NCTC13421_02970)	pilU	3059352..3060470 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DQM71_RS14865 (NCTC13421_02971)	fur	3060540..3060977 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
DQM71_RS14870 (NCTC13421_02972)	-	3061089..3061487 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
DQM71_RS14875 (NCTC13421_02973)	-	3061542..3061865 (-)	324	WP_000442585.1	RnfH family protein	-
DQM71_RS14880 (NCTC13421_02974)	-	3061865..3062932 (-)	1068	WP_000092403.1	hypothetical protein	-
DQM71_RS14890 (NCTC13421_02975)	-	3063085..3063540 (-)	456	WP_000782976.1	bacteriohemerythrin	-
DQM71_RS14895 (NCTC13421_02976)	-	3063793..3064641 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=1142382 DQM71_RS14860 WP_000347036.1 3059352..3060470(+) (pilU) [Acinetobacter baumannii strain NCTC13421]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1142382 DQM71_RS14860 WP_000347036.1 3059352..3060470(+) (pilU) [Acinetobacter baumannii strain NCTC13421]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392