Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DQN50_RS00535 Genome accession   NZ_LS483445
Coordinates   132835..134499 (-) Length   554 a.a.
NCBI ID   WP_003661093.1    Uniprot ID   -
Organism   Moraxella catarrhalis strain NCTC11020     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 127835..139499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN50_RS00515 (NCTC11020_00106) - 129437..129982 (+) 546 WP_003666579.1 DUF924 family protein -
  DQN50_RS00520 (NCTC11020_00107) coaE 129974..130609 (-) 636 WP_003666581.1 dephospho-CoA kinase -
  DQN50_RS00525 (NCTC11020_00108) - 130761..131537 (-) 777 WP_003663394.1 A24 family peptidase -
  DQN50_RS00530 (NCTC11020_00109) - 131537..132796 (-) 1260 WP_063453905.1 type II secretion system F family protein -
  DQN50_RS00535 (NCTC11020_00110) pilB 132835..134499 (-) 1665 WP_003661093.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DQN50_RS00540 (NCTC11020_00111) - 134654..135877 (+) 1224 WP_063453906.1 multidrug effflux MFS transporter -

Sequence

Protein


Download         Length: 554 a.a.        Molecular weight: 60906.14 Da        Isoelectric Point: 6.9938

>NTDB_id=1141769 DQN50_RS00535 WP_003661093.1 132835..134499(-) (pilB) [Moraxella catarrhalis strain NCTC11020]
MTAVLPSLAPLILQHQLMTEDALSKAATDAKNLQRSFVQHLVASGLIDAAKLLHIQSLSYNLPIYEPPSQLSQVVETGLD
PALFIKYRMLPLSCDKHTLTLAMSDATDKDAIGAMRFHARRQIRPMLVEEDKLSALLDRLYLDTDTFEDTQKIGSNSQAT
TDDAPTVGFVNKLFTDAIRLKASDIHFEPYDASYRIRFRIDGVMQVIHNPPKSMAAQIASYIKVMANLDIAERRRAQDGR
LKFDAQDGQSVDFRVSTLPTLHGEKVVLRLLDSAQALIGLDALGMDDAQKQLFIHALSRPQGMILVTGPTGSGKTVSLYT
ALMRLNQPEINILSCEDPVEINLAGINQVNINPKVQLDFADALRAFLRQDPDVIMVGEIRDTDTAQIATTAAQTGHLVLS
TLHTNSASDTITRMHSMGVPSFHLASSLSLIIAQRLARRLCDECKKPITIPDEALRLAGFSDQDIAKQPTLYAPVGCHKC
KNGHKGRIGIFEVLPITPPMAELILTQASAAKIDALARTQGHDNLRQAGIKKVLTGQISLTEMHRVTSESWLNH

Nucleotide


Download         Length: 1665 bp        

>NTDB_id=1141769 DQN50_RS00535 WP_003661093.1 132835..134499(-) (pilB) [Moraxella catarrhalis strain NCTC11020]
ATGACCGCCGTCCTGCCATCACTTGCCCCCCTAATCTTACAACATCAGCTGATGACAGAAGATGCCTTATCAAAAGCTGC
CACTGATGCCAAAAATTTGCAACGCTCATTTGTTCAGCATTTGGTCGCAAGCGGACTCATTGATGCCGCCAAGCTTTTGC
ACATCCAAAGTTTAAGCTACAACTTACCCATTTATGAGCCGCCCAGTCAGCTTAGCCAAGTCGTTGAAACAGGCCTAGAC
CCTGCATTATTTATTAAATATCGAATGCTACCTTTAAGCTGTGATAAGCACACGCTGACCTTAGCTATGAGCGATGCCAC
CGATAAAGATGCCATTGGGGCCATGCGTTTTCATGCACGCCGTCAGATCCGACCTATGCTGGTTGAAGAAGATAAGCTAT
CTGCCTTATTAGATAGGCTATATTTAGATACAGATACTTTTGAGGATACCCAAAAAATAGGGTCAAACTCCCAAGCTACA
ACAGATGACGCCCCCACCGTAGGCTTTGTCAATAAGTTATTCACCGATGCCATCCGCCTAAAAGCATCCGATATTCATTT
TGAGCCTTATGATGCCAGTTATCGCATTCGCTTTCGTATTGATGGGGTAATGCAAGTGATACACAATCCACCCAAATCAA
TGGCCGCTCAGATTGCCTCTTATATCAAAGTCATGGCAAATTTGGATATCGCTGAGCGTCGCCGTGCTCAAGATGGACGC
TTAAAATTTGATGCTCAAGATGGGCAATCGGTGGACTTTCGTGTCAGCACACTGCCTACTTTACATGGTGAAAAGGTCGT
ACTTAGGCTACTGGACTCTGCTCAGGCACTCATTGGGCTAGATGCACTTGGCATGGACGATGCTCAAAAACAGTTATTTA
TTCATGCACTATCACGCCCTCAAGGCATGATTTTGGTCACAGGTCCTACAGGATCAGGTAAGACAGTTTCGCTGTACACA
GCTTTGATGCGTCTTAATCAGCCTGAAATTAATATTTTAAGCTGTGAAGATCCTGTTGAGATTAACTTGGCTGGTATTAA
TCAAGTCAATATTAATCCTAAAGTACAGCTGGATTTTGCCGATGCACTCAGAGCATTTTTACGCCAAGACCCTGATGTCA
TTATGGTTGGGGAAATTCGTGATACTGACACCGCCCAAATCGCCACAACCGCCGCTCAAACGGGGCATTTGGTGTTATCA
ACACTGCACACCAATTCAGCCAGTGATACCATCACGCGTATGCACAGTATGGGTGTGCCATCTTTTCATTTGGCAAGCAG
CTTAAGCCTAATCATCGCCCAAAGATTAGCTCGCCGACTGTGCGATGAATGCAAAAAACCCATCACCATTCCCGATGAGG
CATTACGCTTGGCAGGGTTTAGCGATCAAGATATCGCCAAACAACCAACACTGTACGCACCTGTCGGATGCCATAAATGT
AAAAATGGCCACAAAGGACGCATTGGCATCTTTGAGGTATTACCCATCACGCCGCCCATGGCCGAATTGATTTTAACGCA
GGCATCTGCCGCCAAAATTGATGCTTTGGCACGCACCCAAGGCCATGACAATCTTAGACAAGCAGGCATCAAAAAAGTAC
TCACCGGCCAAATTAGCCTCACTGAGATGCACCGTGTCACCTCTGAATCATGGCTAAATCACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-143)

Type II/IV secretion system protein (T2SSE)

(172-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

48.54

98.917

0.48
  pilB Acinetobacter baumannii D1279779

50.099

91.155

0.457
  pilB Legionella pneumophila strain ERS1305867

45.826

97.292

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

45.506

96.39

0.439
  pilB Vibrio cholerae strain A1552

48.354

87.726

0.424
  pilB Vibrio campbellii strain DS40M4

47.718

87.004

0.415
  pilF Neisseria gonorrhoeae MS11

47.558

85.018

0.404

Multiple sequence alignment