Detailed information NTG1140793

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQN57_RS00695
• Genome accession: NZ_LS483415
• Coordinates: 105934..106368 (+)
• Length: 144 a.a.
• NCBI ID: WP_002986542.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC8304
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 100934..111368

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQN57_RS00665 (NCTC8304_00133)	-	102603..102968 (+)	366	WP_111705425.1	DUF1033 family protein	-
DQN57_RS00670 (NCTC8304_00134)	comYA	103061..103999 (+)	939	WP_002987773.1	competence type IV pilus ATPase ComGA	Machinery gene
DQN57_RS00675 (NCTC8304_00135)	comYB	103935..104969 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQN57_RS00680 (NCTC8304_00136)	comYC	104971..105297 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
DQN57_RS00685 (NCTC8304_00137)	comGD	105272..105700 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
DQN57_RS00690 (NCTC8304_00138)	comGE	105657..105941 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
DQN57_RS00695 (NCTC8304_00139)	comYF	105934..106368 (+)	435	WP_002986542.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQN57_RS00700 (NCTC8304_00140)	comGG	106352..106678 (+)	327	WP_002986539.1	competence type IV pilus minor pilin ComGG	-
DQN57_RS00705 (NCTC8304_00141)	comYH	106776..107729 (+)	954	WP_002987790.1	class I SAM-dependent methyltransferase	Machinery gene
DQN57_RS00710 (NCTC8304_00142)	-	107788..108984 (+)	1197	WP_002986533.1	acetate kinase	-
DQN57_RS00715	-	109171..109479 (+)	309	Protein_90	hypothetical protein	-
DQN57_RS00720 (NCTC8304_00143)	proC	109562..110332 (-)	771	WP_111705426.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16573.20 Da
• Isoelectric Point: 10.3584

>NTDB_id=1140793 DQN57_RS00695 WP_002986542.1 105934..106368(+) (comYF) [Streptococcus pyogenes strain NCTC8304]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1140793 DQN57_RS00695 WP_002986542.1 105934..106368(+) (comYF) [Streptococcus pyogenes strain NCTC8304]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.985	95.139	0.437
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396