Detailed information NTG1140605

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: DQN67_RS04770
• Genome accession: NZ_LS483410
• Coordinates: 1024637..1025701 (+)
• Length: 354 a.a.
• NCBI ID: WP_014843599.1
• Uniprot ID: A0A4T1VG56
• Organism: Legionella pneumophila strain NCTC11404
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1019637..1030701

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQN67_RS04760 (NCTC11404_00935)	-	1020677..1023061 (-)	2385	WP_014843597.1	penicillin-binding protein 1A	-
DQN67_RS04765 (NCTC11404_00936)	-	1023213..1024220 (-)	1008	WP_014843598.1	hypothetical protein	-
DQN67_RS04770 (NCTC11404_00937)	pilM	1024637..1025701 (+)	1065	WP_014843599.1	type IV pilus assembly protein PilM	Machinery gene
DQN67_RS04775 (NCTC11404_00938)	pilN	1025705..1026253 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
DQN67_RS04780 (NCTC11404_00939)	pilO	1026267..1026863 (+)	597	WP_027224065.1	type 4a pilus biogenesis protein PilO	Machinery gene
DQN67_RS04785 (NCTC11404_00940)	pilP	1026860..1027444 (+)	585	WP_014843601.1	pilus assembly protein PilP	Machinery gene
DQN67_RS04790 (NCTC11404_00941)	pilQ	1027448..1029544 (+)	2097	WP_014843602.1	type IV pilus secretin PilQ	Machinery gene
DQN67_RS04795 (NCTC11404_00942)	aroK	1029989..1030516 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38776.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=1140605 DQN67_RS04770 WP_014843599.1 1024637..1025701(+) (pilM) [Legionella pneumophila strain NCTC11404]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLASLIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1140605 DQN67_RS04770 WP_014843599.1 1024637..1025701(+) (pilM) [Legionella pneumophila strain NCTC11404]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCGGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGCCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCATCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A4T1VG56

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407