Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   DQM80_RS00615 Genome accession   NZ_LS483399
Coordinates   98694..99128 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8227     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 93694..104128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM80_RS00585 (NCTC8227_00119) - 95361..95726 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQM80_RS00590 (NCTC8227_00120) comGA 95819..96758 (+) 940 Protein_79 competence type IV pilus ATPase ComGA -
  DQM80_RS00595 (NCTC8227_00121) comGB 96694..97729 (+) 1036 Protein_80 competence type IV pilus assembly protein ComGB -
  DQM80_RS00600 (NCTC8227_00122) comYC 97731..98057 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DQM80_RS00605 (NCTC8227_00123) comGD 98032..98460 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQM80_RS00610 (NCTC8227_00124) comGE 98417..98701 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQM80_RS00615 (NCTC8227_00125) comYF 98694..99128 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQM80_RS00620 (NCTC8227_00126) comGG 99112..99438 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  DQM80_RS00625 (NCTC8227_00127) comYH 99536..100489 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  DQM80_RS00630 (NCTC8227_00128) - 100548..101744 (+) 1197 WP_020904834.1 acetate kinase -
  DQM80_RS00635 - 101931..102239 (+) 309 WP_011017251.1 hypothetical protein -
  DQM80_RS00640 (NCTC8227_00129) proC 102322..103092 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=1140208 DQM80_RS00615 WP_002986542.1 98694..99128(+) (comYF) [Streptococcus pyogenes strain NCTC8227]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1140208 DQM80_RS00615 WP_002986542.1 98694..99128(+) (comYF) [Streptococcus pyogenes strain NCTC8227]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment