Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   DQL29_RS00665 Genome accession   NZ_LS483359
Coordinates   102572..103606 (+) Length   344 a.a.
NCBI ID   WP_227875237.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC12068     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97572..108606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL29_RS00655 (NCTC12068_00133) - 101240..101605 (+) 366 WP_030126461.1 DUF1033 family protein -
  DQL29_RS00660 (NCTC12068_00134) comYA 101698..102636 (+) 939 WP_002993471.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL29_RS00665 (NCTC12068_00135) comYB 102572..103606 (+) 1035 WP_227875237.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL29_RS00670 (NCTC12068_00136) comYC 103608..103934 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL29_RS00675 (NCTC12068_00137) comGD 103909..104337 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQL29_RS00680 (NCTC12068_00138) comGE 104294..104578 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQL29_RS00685 (NCTC12068_00139) comYF 104571..105005 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL29_RS00690 (NCTC12068_00140) comGG 104989..105315 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  DQL29_RS00695 (NCTC12068_00141) comYH 105413..106366 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  DQL29_RS00700 (NCTC12068_00142) - 106425..107621 (+) 1197 WP_030126459.1 acetate kinase -
  DQL29_RS00705 - 107807..108115 (+) 309 WP_030126458.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39692.15 Da        Isoelectric Point: 9.7085

>NTDB_id=1138389 DQL29_RS00665 WP_227875237.1 102572..103606(+) (comYB) [Streptococcus pyogenes strain NCTC12068]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYSREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1138389 DQL29_RS00665 WP_227875237.1 102572..103606(+) (comYB) [Streptococcus pyogenes strain NCTC12068]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATTCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

43.077

94.477

0.407

Multiple sequence alignment