Detailed information NTG1138199

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQL02_RS00685
• Genome accession: NZ_LS483355
• Coordinates: 104536..104970 (+)
• Length: 144 a.a.
• NCBI ID: WP_063510178.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC12067
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99536..109970

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL02_RS00655 (NCTC12067_00132)	-	101205..101570 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQL02_RS00660 (NCTC12067_00133)	comYA	101663..102601 (+)	939	WP_023612548.1	competence type IV pilus ATPase ComGA	Machinery gene
DQL02_RS00665 (NCTC12067_00134)	comYB	102537..103571 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQL02_RS00670 (NCTC12067_00135)	comYC	103573..103899 (+)	327	WP_063510176.1	competence type IV pilus major pilin ComGC	Machinery gene
DQL02_RS00675 (NCTC12067_00136)	comGD	103874..104302 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
DQL02_RS00680 (NCTC12067_00137)	comGE	104259..104543 (+)	285	WP_063510177.1	competence type IV pilus minor pilin ComGE	-
DQL02_RS00685 (NCTC12067_00138)	comYF	104536..104970 (+)	435	WP_063510178.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQL02_RS00690 (NCTC12067_00139)	comGG	104954..105280 (+)	327	WP_002992739.1	competence type IV pilus minor pilin ComGG	-
DQL02_RS00695 (NCTC12067_00140)	comYH	105378..106331 (+)	954	WP_063510179.1	class I SAM-dependent methyltransferase	Machinery gene
DQL02_RS00700 (NCTC12067_00141)	-	106390..107586 (+)	1197	WP_063510180.1	acetate kinase	-
DQL02_RS00705	-	107773..108081 (+)	309	WP_011284425.1	hypothetical protein	-
DQL02_RS00710 (NCTC12067_00142)	proC	108164..108934 (-)	771	WP_011284426.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16504.09 Da
• Isoelectric Point: 10.2532

>NTDB_id=1138199 DQL02_RS00685 WP_063510178.1 104536..104970(+) (comYF) [Streptococcus pyogenes strain NCTC12067]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGASFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1138199 DQL02_RS00685 WP_063510178.1 104536..104970(+) (comYF) [Streptococcus pyogenes strain NCTC12067]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGTTTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.985	95.139	0.437
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396