Detailed information NTG0113711

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P303_RS04995
• Genome accession: NZ_CP006740
• Coordinates: 1087657..1088787 (+)
• Length: 376 a.a.
• NCBI ID: WP_011097971.1
• Uniprot ID: Q87CD3
• Organism: Xylella fastidiosa MUL0034
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1082657..1093787

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P303_RS04975 (P303_05345)	-	1082949..1083773 (+)	825	WP_038229615.1	glycosyltransferase family 2 protein	-
P303_RS04980 (P303_05350)	-	1084403..1085230 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
P303_RS04985 (P303_05355)	-	1085588..1085800 (-)	213	WP_038229612.1	hypothetical protein	-
P303_RS04990 (P303_05360)	pilT	1086457..1087491 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
P303_RS04995 (P303_05365)	pilU	1087657..1088787 (+)	1131	WP_011097971.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
P303_RS05000 (P303_05370)	-	1089480..1090448 (-)	969	WP_004087304.1	YafY family protein	-
P303_RS05005 (P303_05375)	-	1091297..1092106 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
P303_RS05010 (P303_05380)	hemC	1092160..1093077 (-)	918	WP_038229580.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42479.73 Da
• Isoelectric Point: 6.5563

>NTDB_id=113711 P303_RS04995 WP_011097971.1 1087657..1088787(+) (pilU) [Xylella fastidiosa MUL0034]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=113711 P303_RS04995 WP_011097971.1 1087657..1088787(+) (pilU) [Xylella fastidiosa MUL0034]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

3D structure

• AlphaFold DB: Q87CD3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	50.843	94.681	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402