Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   DQL28_RS00665 Genome accession   NZ_LS483334
Coordinates   102425..103459 (+) Length   344 a.a.
NCBI ID   WP_227868552.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC12050     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97425..108459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL28_RS00655 (NCTC12050_00131) - 101093..101458 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQL28_RS00660 (NCTC12050_00132) comYA 101551..102489 (+) 939 WP_011284420.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL28_RS00665 (NCTC12050_00133) comYB 102425..103459 (+) 1035 WP_227868552.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL28_RS00670 (NCTC12050_00134) comYC 103461..103787 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL28_RS00675 (NCTC12050_00135) comGD 103762..104190 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQL28_RS00680 (NCTC12050_00136) comGE 104147..104431 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQL28_RS00685 (NCTC12050_00137) comYF 104424..104858 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL28_RS00690 (NCTC12050_00138) comGG 104842..105168 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  DQL28_RS00695 (NCTC12050_00139) comYH 105266..106219 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  DQL28_RS00700 (NCTC12050_00140) - 106278..107474 (+) 1197 WP_109821056.1 acetate kinase -
  DQL28_RS00705 - 107661..107969 (+) 309 Protein_89 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39706.18 Da        Isoelectric Point: 9.7085

>NTDB_id=1137057 DQL28_RS00665 WP_227868552.1 102425..103459(+) (comYB) [Streptococcus pyogenes strain NCTC12050]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEIITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYSREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1137057 DQL28_RS00665 WP_227868552.1 102425..103459(+) (comYB) [Streptococcus pyogenes strain NCTC12050]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAATTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATTCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.392

98.547

0.526
  comYB Streptococcus mutans UA159

53.392

98.547

0.526
  comYB Streptococcus gordonii str. Challis substr. CH1

50

98.837

0.494
  comGB/cglB Streptococcus mitis NCTC 12261

47.024

97.674

0.459
  comGB/cglB Streptococcus mitis SK321

47.147

96.802

0.456
  comGB/cglB Streptococcus pneumoniae Rx1

45.045

96.802

0.436
  comGB/cglB Streptococcus pneumoniae D39

45.045

96.802

0.436
  comGB/cglB Streptococcus pneumoniae R6

45.045

96.802

0.436
  comGB/cglB Streptococcus pneumoniae TIGR4

45.045

96.802

0.436
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment