Detailed information NTG0113579

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: D934_RS06445
• Genome accession: NZ_CP006696
• Coordinates: 1414963..1416093 (-)
• Length: 376 a.a.
• NCBI ID: WP_020851681.1
• Uniprot ID: A0A060H3A3
• Organism: Xylella fastidiosa subsp. sandyi Ann-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1409963..1421093

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D934_RS06430 (D934_06895)	hemC	1410695..1411612 (+)	918	WP_020851685.1	hydroxymethylbilane synthase	-
D934_RS06435 (D934_06900)	-	1411666..1412475 (-)	810	WP_021358437.1	DUF481 domain-containing protein	-
D934_RS06440 (D934_06905)	-	1413324..1414293 (+)	970	Protein_1262	YafY family protein	-
D934_RS06445 (D934_06910)	pilU	1414963..1416093 (-)	1131	WP_020851681.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
D934_RS06450 (D934_06915)	pilT	1416259..1417293 (-)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
D934_RS06455 (D934_06920)	-	1417967..1418179 (+)	213	WP_024749215.1	hypothetical protein	-
D934_RS06465 (D934_06930)	-	1418527..1419354 (+)	828	WP_020851677.1	glycosyltransferase family 2 protein	-
D934_RS14410 (D934_06935)	-	1419495..1419749 (+)	255	WP_024749217.1	hypothetical protein	-
D934_RS06475 (D934_06940)	-	1420030..1420854 (-)	825	WP_020851675.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42434.64 Da
• Isoelectric Point: 6.3406

>NTDB_id=113579 D934_RS06445 WP_020851681.1 1414963..1416093(-) (pilU) [Xylella fastidiosa subsp. sandyi Ann-1]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERHSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEILRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAQTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=113579 D934_RS06445 WP_020851681.1 1414963..1416093(-) (pilU) [Xylella fastidiosa subsp. sandyi Ann-1]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCACAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAATTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTCAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

3D structure

• AlphaFold DB: A0A060H3A3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.308	97.606	0.598
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	50.281	94.681	0.476
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.758	96.809	0.404