Detailed information NTG1134416

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JMW66_RS02020
• Genome accession: NZ_LR890619
• Coordinates: 436459..437607 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain MINF_7A isolate MINF_7A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 431459..442607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JMW66_RS01995	-	432610..432906 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
JMW66_RS02000	-	432903..433496 (-)	594	WP_003084544.1	YggT family protein	-
JMW66_RS02005	proC	433507..434328 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
JMW66_RS02010	-	434340..435032 (-)	693	WP_201522582.1	YggS family pyridoxal phosphate-dependent enzyme	-
JMW66_RS02015	pilT	435247..436281 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
JMW66_RS02020	pilU	436459..437607 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
JMW66_RS02025	czcD	437615..438514 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
JMW66_RS02030	-	438668..439072 (+)	405	WP_003084560.1	NINE protein	-
JMW66_RS02035	-	439315..440688 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
JMW66_RS02040	-	440685..441869 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1134416 JMW66_RS02020 WP_003100959.1 436459..437607(+) (pilU) [Pseudomonas aeruginosa strain MINF_7A isolate MINF_7A]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1134416 JMW66_RS02020 WP_003100959.1 436459..437607(+) (pilU) [Pseudomonas aeruginosa strain MINF_7A isolate MINF_7A]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382