Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   PPECC33_RS17960 Genome accession   NZ_CP006632
Coordinates   3643844..3644968 (-) Length   374 a.a.
NCBI ID   WP_000228539.1    Uniprot ID   A0A368IM01
Organism   Escherichia coli PCN033     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3638844..3649968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPECC33_RS17930 (PPECC33_03597) yrdA 3640644..3641198 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  PPECC33_RS17935 (PPECC33_03598) yrdB 3641174..3641431 (-) 258 WP_001070567.1 DUF1488 domain-containing protein -
  PPECC33_RS17940 (PPECC33_03599) aroE 3641428..3642246 (-) 819 WP_000451232.1 shikimate dehydrogenase -
  PPECC33_RS17945 (PPECC33_03600) tsaC 3642251..3642823 (-) 573 WP_001366671.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  PPECC33_RS17950 yrdD 3642828..3643370 (-) 543 WP_001129723.1 type I DNA topoisomerase -
  PPECC33_RS17955 (PPECC33_03602) smg 3643399..3643872 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  PPECC33_RS17960 (PPECC33_03603) dprA 3643844..3644968 (-) 1125 WP_000228539.1 DNA-protecting protein DprA Machinery gene
  PPECC33_RS17965 (PPECC33_03604) def 3645098..3645607 (+) 510 WP_000114984.1 peptide deformylase -
  PPECC33_RS17970 (PPECC33_03605) fmt 3645622..3646569 (+) 948 WP_000004443.1 methionyl-tRNA formyltransferase -
  PPECC33_RS17975 (PPECC33_03606) rsmB 3646615..3647904 (+) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  PPECC33_RS17980 (PPECC33_03607) trkA 3647926..3649302 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  PPECC33_RS17985 (PPECC33_03608) mscL 3649432..3649842 (+) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40976.96 Da        Isoelectric Point: 6.3122

>NTDB_id=113266 PPECC33_RS17960 WP_000228539.1 3643844..3644968(-) (dprA) [Escherichia coli PCN033]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSYIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVAGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=113266 PPECC33_RS17960 WP_000228539.1 3643844..3644968(-) (dprA) [Escherichia coli PCN033]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGTATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGCAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCGCGTCGCCATGCCCGACTGGCTACCAGTTTGCT
TGAACATGGTGGGGCTCTTGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTTTAAGTAAAGGTGTACTAGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGACCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A368IM01

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

48.71

82.888

0.404
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

44.377

87.968

0.39

Multiple sequence alignment