Detailed information NTG1129732

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PERCYII40_RS27065
• Genome accession: NZ_LR739071
• Coordinates: 5869384..5870448 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain C7-25 isolate C7-25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5864384..5875448

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PERCYII40_RS27040 (PACF725_5403)	aroK	5864919..5865437 (-)	519	WP_003095833.1	shikimate kinase AroK	-
PERCYII40_RS27045 (PACF725_5404)	pilQ	5865449..5867593 (-)	2145	WP_012614629.1	type 4a pilus secretin PilQ	Machinery gene
PERCYII40_RS27050 (PACF725_5405)	pilP	5867647..5868171 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
PERCYII40_RS27055 (PACF725_5406)	pilO	5868168..5868791 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
PERCYII40_RS27060 (PACF725_5407)	pilN	5868788..5869384 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
PERCYII40_RS27065 (PACF725_5408)	pilM	5869384..5870448 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
PERCYII40_RS27070 (PACF725_5409)	-	5870633..5873101 (+)	2469	WP_003114576.1	penicillin-binding protein 1A	-
PERCYII40_RS27075 (PACF725_5410)	-	5873206..5874474 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=1129732 PERCYII40_RS27065 WP_003110888.1 5869384..5870448(-) (pilM) [Pseudomonas aeruginosa strain C7-25 isolate C7-25]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1129732 PERCYII40_RS27065 WP_003110888.1 5869384..5870448(-) (pilM) [Pseudomonas aeruginosa strain C7-25 isolate C7-25]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACGCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466