Detailed information NTG1129636

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: G3R42_RS02035
• Genome accession: NZ_LR739068
• Coordinates: 443215..444363 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PcyII-29 isolate PcyII-29
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 438215..449363

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G3R42_RS02010	-	439366..439662 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
G3R42_RS02015 (PERCYII29_0403)	-	439659..440252 (-)	594	WP_003084544.1	YggT family protein	-
G3R42_RS02020 (PERCYII29_0404)	proC	440263..441084 (-)	822	WP_003100962.1	pyrroline-5-carboxylate reductase	-
G3R42_RS02025 (PERCYII29_0405)	-	441096..441788 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
G3R42_RS02030 (PERCYII29_0406)	pilT	442003..443037 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
G3R42_RS02035 (PERCYII29_0407)	pilU	443215..444363 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
G3R42_RS02040 (PERCYII29_0408)	czcD	444371..445270 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
G3R42_RS02045 (PERCYII29_0409)	-	445424..445828 (+)	405	WP_003084560.1	NINE protein	-
G3R42_RS02050 (PERCYII29_0410)	-	446071..447444 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
G3R42_RS02055 (PERCYII29_0411)	-	447441..448625 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1129636 G3R42_RS02035 WP_003100959.1 443215..444363(+) (pilU) [Pseudomonas aeruginosa strain PcyII-29 isolate PcyII-29]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1129636 G3R42_RS02035 WP_003100959.1 443215..444363(+) (pilU) [Pseudomonas aeruginosa strain PcyII-29 isolate PcyII-29]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGTGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382