Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   GPW68_RS00820 Genome accession   NZ_LR738723
Coordinates   124331..124765 (+) Length   144 a.a.
NCBI ID   WP_074389248.1    Uniprot ID   A0AAP6A8C9
Organism   Streptococcus suis isolate GD-0088     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 125634..146566 124331..124765 flank 869


Gene organization within MGE regions


Location: 124331..146566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW68_RS00820 comGF/cglF 124331..124765 (+) 435 WP_074389248.1 competence type IV pilus minor pilin ComGF Machinery gene
  GPW68_RS00825 comGG 124743..125153 (+) 411 WP_074389249.1 competence type IV pilus minor pilin ComGG -
  GPW68_RS00830 - 125634..126059 (-) 426 WP_226319165.1 ArpU family phage packaging/lysis transcriptional regulator -
  GPW68_RS00835 - 126383..126958 (-) 576 WP_074392204.1 hypothetical protein -
  GPW68_RS11485 - 126979..127152 (-) 174 WP_171840772.1 hypothetical protein -
  GPW68_RS00840 - 127466..128902 (-) 1437 WP_156721189.1 phage/plasmid primase, P4 family -
  GPW68_RS00845 - 128965..129843 (-) 879 WP_074403919.1 primase alpha helix C-terminal domain-containing protein -
  GPW68_RS00850 - 129978..130262 (-) 285 WP_044766582.1 hypothetical protein -
  GPW68_RS00855 - 130252..130590 (-) 339 WP_074390405.1 hypothetical protein -
  GPW68_RS00865 - 130807..131250 (-) 444 WP_074390406.1 hypothetical protein -
  GPW68_RS00870 - 131722..132348 (-) 627 WP_074390407.1 Rha family transcriptional regulator -
  GPW68_RS00875 - 132365..132565 (-) 201 WP_015647585.1 helix-turn-helix transcriptional regulator -
  GPW68_RS11570 - 132751..133260 (+) 510 WP_370695883.1 helix-turn-helix domain-containing protein -
  GPW68_RS00885 - 133721..134866 (+) 1146 WP_074390408.1 tyrosine-type recombinase/integrase -
  GPW68_RS00890 comYH 134963..135916 (+) 954 WP_074389250.1 class I SAM-dependent methyltransferase Machinery gene
  GPW68_RS00895 - 135966..137153 (+) 1188 WP_074389251.1 acetate kinase -
  GPW68_RS00900 - 137468..138019 (+) 552 WP_024389479.1 folate family ECF transporter S component -
  GPW68_RS00905 - 138075..139331 (+) 1257 WP_074389253.1 bifunctional folylpolyglutamate synthase/dihydrofolate synthase -
  GPW68_RS00910 pepA 139376..140437 (-) 1062 WP_074389254.1 glutamyl aminopeptidase -
  GPW68_RS00915 - 140584..140868 (+) 285 WP_044674517.1 DUF4651 domain-containing protein -
  GPW68_RS00920 - 140865..141185 (+) 321 WP_024386832.1 thioredoxin family protein -
  GPW68_RS00925 - 141230..142186 (+) 957 WP_044754802.1 DUF1002 domain-containing protein -
  GPW68_RS00930 ytpR 142205..142828 (+) 624 WP_074389255.1 YtpR family tRNA-binding protein -
  GPW68_RS00935 - 142861..143640 (-) 780 WP_074389256.1 DUF2785 domain-containing protein -
  GPW68_RS00940 ssbA 143695..144090 (+) 396 WP_011921737.1 single-stranded DNA-binding protein Machinery gene
  GPW68_RS11490 - 144209..144454 (-) 246 WP_074389257.1 hypothetical protein -
  GPW68_RS00950 groES 144651..144932 (+) 282 WP_074389258.1 co-chaperone GroES -
  GPW68_RS00955 groL 144944..146566 (+) 1623 WP_074389259.1 chaperonin GroEL -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16432.74 Da        Isoelectric Point: 6.9832

>NTDB_id=1129532 GPW68_RS00820 WP_074389248.1 124331..124765(+) (comGF/cglF) [Streptococcus suis isolate GD-0088]
MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAVSQENQLVRIDFNFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1129532 GPW68_RS00820 WP_074389248.1 124331..124765(+) (comGF/cglF) [Streptococcus suis isolate GD-0088]
TTGTTAAAAAGTAAAATTCCTGCCTTTACTCTCTTGGAATGCTTGGTAGCCTTAGTCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGTTACCAGCCCATGCT
CTATCAGGTGGATAGCGCAGCCGTTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAACTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae D39

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae R6

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae TIGR4

52.482

97.917

0.514
  comYF Streptococcus mutans UA140

50

100

0.5
  comYF Streptococcus mutans UA159

49.306

100

0.493
  comGF/cglF Streptococcus mitis NCTC 12261

52.985

93.056

0.493
  comGF/cglF Streptococcus mitis SK321

50

93.056

0.465
  comGF Lactococcus lactis subsp. cremoris KW2

46.099

97.917

0.451

Multiple sequence alignment