Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   FGL04_RS02800 Genome accession   NZ_LR594035
Coordinates   509127..510377 (+) Length   416 a.a.
NCBI ID   WP_225247757.1    Uniprot ID   A0A4U9XQZ2
Organism   Streptococcus pseudoporcinus strain NCTC5385     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 507170..559394 509127..510377 within 0
IScluster/Tn 508007..512219 509127..510377 within 0


Gene organization within MGE regions


Location: 507170..559394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL04_RS02790 (NCTC5385_00586) - 507170..507799 (-) 630 WP_077323440.1 YigZ family protein -
  FGL04_RS02795 (NCTC5385_00588) - 508007..509112 (+) 1106 WP_138068165.1 IS3 family transposase -
  FGL04_RS02800 (NCTC5385_00589) comFA/cflA 509127..510377 (+) 1251 WP_225247757.1 DEAD/DEAH box helicase Machinery gene
  FGL04_RS02805 (NCTC5385_00590) - 510367..510870 (+) 504 WP_225247758.1 ComF family protein -
  FGL04_RS10895 - 510907..511047 (+) 141 WP_225247843.1 phosphoribosyltransferase family protein -
  FGL04_RS02810 (NCTC5385_00591) - 511086..512219 (-) 1134 Protein_532 IS3 family transposase -
  FGL04_RS02815 (NCTC5385_00593) - 512299..512472 (+) 174 Protein_533 ParA family protein -
  FGL04_RS02820 (NCTC5385_00594) - 512472..512717 (+) 246 WP_138068167.1 hypothetical protein -
  FGL04_RS02825 - 512761..513144 (+) 384 Protein_535 replication initiator protein A -
  FGL04_RS02830 (NCTC5385_00596) - 513148..513507 (+) 360 WP_138068168.1 hypothetical protein -
  FGL04_RS02835 (NCTC5385_00597) - 513491..513760 (+) 270 WP_138068169.1 hypothetical protein -
  FGL04_RS02840 (NCTC5385_00598) - 513924..515219 (+) 1296 WP_225247844.1 ISLre2 family transposase -
  FGL04_RS02875 (NCTC5385_00606) - 521454..521768 (-) 315 WP_138068170.1 DUF960 domain-containing protein -
  FGL04_RS02880 (NCTC5385_00607) - 521830..522363 (-) 534 WP_138068171.1 DUF402 domain-containing protein -
  FGL04_RS02885 (NCTC5385_00608) recX 522444..523220 (-) 777 WP_171011267.1 recombination regulator RecX -
  FGL04_RS02890 - 523321..524075 (-) 755 Protein_542 IS3 family transposase -
  FGL04_RS02900 - 525294..525687 (-) 394 Protein_544 transposase -
  FGL04_RS02905 - 525767..525949 (+) 183 Protein_545 DNA topoisomerase III -
  FGL04_RS10695 (NCTC5385_00614) - 526030..526167 (+) 138 Protein_546 AAA family ATPase -
  FGL04_RS02910 (NCTC5385_00615) - 526187..527098 (-) 912 Protein_547 IS3 family transposase -
  FGL04_RS02915 (NCTC5385_00617) - 527175..528280 (+) 1106 WP_138068165.1 IS3 family transposase -
  FGL04_RS02920 (NCTC5385_00618) - 528322..528552 (-) 231 Protein_549 transposase -
  FGL04_RS02925 (NCTC5385_00619) - 528632..529189 (+) 558 Protein_550 hypothetical protein -
  FGL04_RS02930 (NCTC5385_00620) - 529242..530474 (+) 1233 WP_138068173.1 MFS transporter -
  FGL04_RS02935 (NCTC5385_00621) - 530491..531366 (+) 876 WP_138068174.1 shikimate dehydrogenase -
  FGL04_RS02940 (NCTC5385_00622) - 531913..532521 (+) 609 WP_138068175.1 VTT domain-containing protein -
  FGL04_RS02945 (NCTC5385_00623) - 532836..533099 (+) 264 WP_225247759.1 hypothetical protein -
  FGL04_RS02955 (NCTC5385_00626) - 534675..536519 (+) 1845 WP_138068176.1 glycerophosphoryl diester phosphodiesterase membrane domain-containing protein -
  FGL04_RS02960 (NCTC5385_00627) - 536850..537248 (-) 399 Protein_557 DDE-type integrase/transposase/recombinase -
  FGL04_RS02965 (NCTC5385_00629) - 537315..538420 (+) 1106 WP_138068165.1 IS3 family transposase -
  FGL04_RS10820 (NCTC5385_00630) - 538447..538826 (-) 380 Protein_559 IS3 family transposase -
  FGL04_RS02980 (NCTC5385_00633) - 540043..540302 (-) 260 Protein_561 transposase -
  FGL04_RS02995 (NCTC5385_00634) - 540861..541178 (+) 318 WP_225247845.1 tyrosine-type recombinase/integrase -
  FGL04_RS03005 (NCTC5385_00636) - 541651..543021 (-) 1371 WP_138068179.1 MATE family efflux transporter -
  FGL04_RS03010 (NCTC5385_00637) - 543033..543467 (-) 435 WP_138068180.1 MarR family winged helix-turn-helix transcriptional regulator -
  FGL04_RS03015 (NCTC5385_00638) - 543686..543940 (+) 255 WP_077322853.1 DUF1912 family protein -
  FGL04_RS03020 (NCTC5385_00640) - 544431..544976 (+) 546 Protein_566 GNAT family N-acetyltransferase -
  FGL04_RS03025 (NCTC5385_00641) - 545012..545578 (+) 567 WP_138068181.1 ATP-binding protein -
  FGL04_RS03030 (NCTC5385_00642) - 545649..548300 (+) 2652 WP_138068182.1 valine--tRNA ligase -
  FGL04_RS03035 tnpA 548575..549053 (+) 479 Protein_569 IS200/IS605 family transposase -
  FGL04_RS11335 (NCTC5385_00646) comE/blpR 549478..549681 (+) 204 WP_181950250.1 hypothetical protein Regulator
  FGL04_RS03050 (NCTC5385_00648) - 549744..550849 (+) 1106 WP_138068165.1 IS3 family transposase -
  FGL04_RS03055 - 550896..551057 (+) 162 WP_225247760.1 hypothetical protein -
  FGL04_RS11245 - 551548..551670 (+) 123 WP_269902558.1 hypothetical protein -
  FGL04_RS03070 (NCTC5385_00649) mutM 552002..552823 (+) 822 WP_138068183.1 DNA-formamidopyrimidine glycosylase -
  FGL04_RS03075 (NCTC5385_00650) coaE 552820..553425 (+) 606 WP_138068184.1 dephospho-CoA kinase -
  FGL04_RS03080 (NCTC5385_00651) - 553730..555229 (+) 1500 WP_138068185.1 helicase HerA-like domain-containing protein -
  FGL04_RS03085 (NCTC5385_00652) - 555361..556530 (+) 1170 WP_333473201.1 multidrug efflux MFS transporter -
  FGL04_RS03090 rpmG 556527..556673 (+) 147 WP_077323081.1 50S ribosomal protein L33 -
  FGL04_RS03095 (NCTC5385_00653) secG 556719..556955 (+) 237 WP_007895702.1 preprotein translocase subunit SecG -
  FGL04_RS03100 (NCTC5385_00654) rnr 557043..559394 (+) 2352 WP_138068187.1 ribonuclease R -

Sequence

Protein


Download         Length: 416 a.a.        Molecular weight: 47360.08 Da        Isoelectric Point: 9.7867

>NTDB_id=1127288 FGL04_RS02800 WP_225247757.1 509127..510377(+) (comFA/cflA) [Streptococcus pseudoporcinus strain NCTC5385]
MLYRKVLPFQKDSFEGDGKAYYCGRCLSKAGKDHLLPNEHYYCRQCLVFGRVQSHDKLYYFPAPPFLKGNYLKWKGSLTP
YQESISKQLVENYYLGKWSLVYAVTGAGKTEMIYSVIKEVVNSGKWVALVSPRVDVCIEVYKRLSRDFSCQTILMHAGSE
SYHRAPLVIATTHQLLKFYKAFHLIIIDEVDSFPFVDNPLLNRAVMSALKEEGQLIYLTATSTKFLESEVTKGKLIKLTL
PRRFHNNPLIVPKFQLILNFQNYLDKGKLPTKLYSAIQKQTSLPYPLLIFYPVIEKGQMFYDSLVKSFPNHQIGYVSSQT
AKRKELIEDFRQGSLSILVTTTILERGVTFPGADVFVVLAHHHLFTSSSLIQIAGSVGRSVDRPNGKVIFFHEGVSSAMY
KARKEIIALNKEAYGS

Nucleotide


Download         Length: 1251 bp        

>NTDB_id=1127288 FGL04_RS02800 WP_225247757.1 509127..510377(+) (comFA/cflA) [Streptococcus pseudoporcinus strain NCTC5385]
ATGTTGTACAGAAAAGTGTTGCCTTTTCAGAAAGATAGTTTTGAGGGTGATGGTAAGGCTTATTATTGCGGACGTTGTCT
CAGTAAAGCTGGTAAAGATCACTTACTTCCTAATGAGCATTATTATTGTAGACAATGTTTAGTTTTTGGACGAGTGCAAA
GTCATGATAAACTCTATTATTTTCCTGCTCCGCCATTTTTAAAAGGAAATTATCTCAAGTGGAAAGGAAGCTTAACTCCT
TATCAAGAGAGTATTTCTAAACAATTAGTTGAAAATTATTATTTAGGAAAATGGAGTCTGGTTTATGCTGTTACAGGGGC
AGGTAAAACGGAGATGATTTATAGCGTTATCAAAGAAGTAGTCAATAGTGGGAAATGGGTTGCTTTAGTCAGTCCGAGAG
TTGATGTCTGTATTGAAGTCTATAAACGCTTAAGCCGTGATTTCTCATGTCAGACTATTTTGATGCATGCGGGGTCAGAA
AGTTATCATAGAGCTCCGCTTGTTATTGCCACAACCCACCAGTTATTGAAGTTTTATAAGGCATTTCATTTGATTATTAT
TGATGAGGTTGATTCTTTTCCCTTTGTTGATAATCCCCTCCTAAATCGTGCTGTTATGTCAGCATTGAAAGAGGAAGGTC
AGTTAATCTATTTGACAGCCACATCCACGAAGTTTTTAGAAAGTGAGGTGACCAAAGGGAAACTGATCAAATTAACACTT
CCAAGACGATTTCATAATAATCCATTAATAGTGCCTAAATTTCAATTGATTTTAAACTTTCAAAACTATTTAGATAAAGG
TAAACTCCCTACCAAACTATACTCTGCCATCCAAAAGCAGACTTCTTTGCCATATCCTCTTTTGATTTTTTATCCTGTCA
TAGAGAAAGGTCAAATGTTCTATGATAGTTTAGTAAAATCATTTCCTAATCACCAAATAGGCTATGTAAGTAGTCAAACG
GCTAAACGTAAAGAATTGATTGAGGACTTTCGTCAAGGGAGTCTGTCGATTCTTGTTACGACTACTATACTAGAAAGAGG
TGTTACTTTTCCTGGGGCAGATGTCTTTGTTGTATTAGCGCATCATCACCTTTTTACGTCTTCAAGTCTCATTCAAATAG
CAGGGAGTGTTGGGAGATCCGTTGATAGACCAAATGGTAAGGTAATATTTTTCCATGAGGGAGTGAGCTCTGCTATGTAT
AAAGCTAGAAAAGAAATAATAGCTTTAAATAAGGAAGCTTATGGATCATAA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(84-224)

Helicase conserved C-terminal domain (Helicase_C)

(273-379)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U9XQZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae D39

56.122

94.231

0.529
  comFA/cflA Streptococcus pneumoniae R6

56.122

94.231

0.529
  comFA/cflA Streptococcus pneumoniae TIGR4

56.122

94.231

0.529
  comFA/cflA Streptococcus pneumoniae Rx1

56.122

94.231

0.529
  comFA/cflA Streptococcus mitis SK321

54.847

94.231

0.517
  comFA/cflA Streptococcus mitis NCTC 12261

54.592

94.231

0.514
  comFA Lactococcus lactis subsp. cremoris KW2

46.667

93.75

0.438
  comFA Latilactobacillus sakei subsp. sakei 23K

38.325

94.712

0.363

Multiple sequence alignment