Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   E0F40_RS10315 Genome accession   NZ_LR216065
Coordinates   1903027..1904043 (-) Length   338 a.a.
NCBI ID   WP_077141332.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1898027..1909043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F40_RS10285 (SAMEA3714487_01960) - 1898175..1898465 (-) 291 WP_001815531.1 hypothetical protein -
  E0F40_RS11820 - 1898462..1898608 (-) 147 WP_000389580.1 hypothetical protein -
  E0F40_RS12365 (SAMEA3714487_01961) - 1899003..1899125 (-) 123 WP_000343850.1 hypothetical protein -
  E0F40_RS10290 (SAMEA3714487_01962) - 1899199..1899474 (-) 276 WP_001094372.1 hypothetical protein -
  E0F40_RS10295 (SAMEA3714487_01963) - 1899649..1900455 (+) 807 WP_000090700.1 XRE family transcriptional regulator -
  E0F40_RS10300 (SAMEA3714487_01964) - 1900457..1901221 (+) 765 WP_000032361.1 type II toxin-antitoxin system PemK/MazF family toxin -
  E0F40_RS10305 (SAMEA3714487_01965) - 1901500..1902945 (+) 1446 WP_050105991.1 recombinase family protein -
  E0F40_RS10315 (SAMEA3714487_01966) comGB/cglB 1903027..1904043 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F40_RS10320 (SAMEA3714487_01967) comGA/cglA/cilD 1903991..1904932 (-) 942 WP_000249550.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F40_RS10325 (SAMEA3714487_01968) - 1905008..1905373 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F40_RS10330 (SAMEA3714487_01969) - 1905524..1906582 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F40_RS10335 (SAMEA3714487_01970) nagA 1906745..1907896 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38466.48 Da        Isoelectric Point: 9.6162

>NTDB_id=1126375 E0F40_RS10315 WP_077141332.1 1903027..1904043(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1126375 E0F40_RS10315 WP_077141332.1 1903027..1904043(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTTATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment