Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   EL300_RS10735 Genome accession   NZ_LR134532
Coordinates   2268453..2269787 (+) Length   444 a.a.
NCBI ID   WP_016533996.1    Uniprot ID   -
Organism   Pasteurella multocida strain NCTC10323     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2263453..2274787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL300_RS10710 (NCTC10323_02180) - 2263529..2266090 (-) 2562 WP_005751905.1 penicillin-binding protein 1A -
  EL300_RS10715 (NCTC10323_02181) - 2266226..2266999 (+) 774 WP_005734313.1 pilus assembly protein PilM -
  EL300_RS10720 (NCTC10323_02182) - 2267039..2267554 (+) 516 WP_005751904.1 hypothetical protein -
  EL300_RS10725 (NCTC10323_02183) - 2267554..2268078 (+) 525 WP_005751903.1 hypothetical protein -
  EL300_RS10730 (NCTC10323_02184) - 2268081..2268443 (+) 363 WP_005748555.1 pilus assembly protein PilP -
  EL300_RS10735 (NCTC10323_02185) comE 2268453..2269787 (+) 1335 WP_016533996.1 type IV pilus secretin PilQ Machinery gene
  EL300_RS10740 (NCTC10323_02186) aroK 2269970..2270497 (+) 528 WP_005717623.1 shikimate kinase AroK -
  EL300_RS10745 (NCTC10323_02187) aroB 2270514..2271602 (+) 1089 WP_005751902.1 3-dehydroquinate synthase -
  EL300_RS10750 (NCTC10323_02188) - 2271606..2272511 (+) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EL300_RS10755 (NCTC10323_02189) - 2272558..2272863 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  EL300_RS10760 (NCTC10323_02190) secM 2272956..2273267 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49266.72 Da        Isoelectric Point: 8.4317

>NTDB_id=1124103 EL300_RS10735 WP_016533996.1 2268453..2269787(+) (comE) [Pasteurella multocida strain NCTC10323]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEKIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKY
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=1124103 EL300_RS10735 WP_016533996.1 2268453..2269787(+) (comE) [Pasteurella multocida strain NCTC10323]
ATGTGGAGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAAAAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAATAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCAAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.806

97.297

0.601
  comE Haemophilus influenzae 86-028NP

61.111

97.297

0.595
  comE Glaesserella parasuis strain SC1401

48.73

97.523

0.475
  pilQ Vibrio campbellii strain DS40M4

39.286

94.595

0.372
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.563

92.793

0.367
  pilQ Vibrio cholerae strain A1552

39.563

92.793

0.367

Multiple sequence alignment