Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   EL252_RS10635 Genome accession   NZ_LR134514
Coordinates   2252346..2253680 (+) Length   444 a.a.
NCBI ID   WP_126417845.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. septica strain NCTC11619     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2247346..2258680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL252_RS10610 (NCTC11619_02136) - 2247422..2249983 (-) 2562 WP_126417842.1 penicillin-binding protein 1A -
  EL252_RS10615 (NCTC11619_02137) - 2250119..2250892 (+) 774 WP_126417843.1 pilus assembly protein PilM -
  EL252_RS10620 (NCTC11619_02138) - 2250932..2251447 (+) 516 WP_104884245.1 competence protein ComB -
  EL252_RS10625 (NCTC11619_02139) - 2251447..2251971 (+) 525 WP_083003382.1 ATPase -
  EL252_RS10630 (NCTC11619_02140) - 2251974..2252336 (+) 363 WP_126417844.1 pilus assembly protein PilP -
  EL252_RS10635 (NCTC11619_02141) comE 2252346..2253680 (+) 1335 WP_126417845.1 type IV pilus secretin PilQ Machinery gene
  EL252_RS10640 (NCTC11619_02142) aroK 2253863..2254390 (+) 528 WP_005717623.1 shikimate kinase AroK -
  EL252_RS10645 (NCTC11619_02143) aroB 2254407..2255495 (+) 1089 WP_126417846.1 3-dehydroquinate synthase -
  EL252_RS10650 (NCTC11619_02144) - 2255499..2256404 (+) 906 WP_083003372.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EL252_RS10655 (NCTC11619_02145) - 2256451..2256756 (-) 306 WP_083003370.1 DUF721 domain-containing protein -
  EL252_RS10660 (NCTC11619_02146) secM 2256849..2257160 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49222.53 Da        Isoelectric Point: 7.1842

>NTDB_id=1123680 EL252_RS10635 WP_126417845.1 2252346..2253680(+) (comE) [Pasteurella multocida subsp. septica strain NCTC11619]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSITLQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQETERHQKRELVIFVTPHIIKPTQSSPEQKTTGVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=1123680 EL252_RS10635 WP_126417845.1 2252346..2253680(+) (comE) [Pasteurella multocida subsp. septica strain NCTC11619]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTATTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAATTCCATCAAGAAGGGAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCACCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGATAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAGGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGGAAACTGAACGTCATCAAAAGCGAGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCGACCCA
AAGTTCGCCTGAACAAAAAACAACGGGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.348

100

0.615
  comE Haemophilus influenzae 86-028NP

60.674

100

0.608
  comE Glaesserella parasuis strain SC1401

48.38

97.297

0.471
  pilQ Vibrio campbellii strain DS40M4

39.286

94.595

0.372
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.563

92.793

0.367
  pilQ Vibrio cholerae strain A1552

39.563

92.793

0.367

Multiple sequence alignment