Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   I927_RS08055 Genome accession   NZ_CP004392
Coordinates   1739883..1741217 (+) Length   444 a.a.
NCBI ID   WP_046333760.1    Uniprot ID   -
Organism   Pasteurella multocida OH1905     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1705413..1741217 1739883..1741217 within 0


Gene organization within MGE regions


Location: 1705413..1741217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I927_RS07865 (I927_07855) - 1705413..1706093 (-) 681 WP_046333738.1 YtjB family periplasmic protein -
  I927_RS07870 (I927_07860) serB 1706173..1707135 (+) 963 WP_046333739.1 phosphoserine phosphatase -
  I927_RS07875 (I927_07865) - 1707142..1707633 (+) 492 WP_005718544.1 YajQ family cyclic di-GMP-binding protein -
  I927_RS07880 (I927_07870) - 1707723..1707986 (-) 264 WP_005718542.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  I927_RS07885 (I927_07875) - 1708432..1708896 (-) 465 WP_080940663.1 integrase arm-type DNA-binding domain-containing protein -
  I927_RS07890 (I927_07880) - 1709375..1710292 (-) 918 WP_046333740.1 hypothetical protein -
  I927_RS07895 (I927_07885) - 1710292..1710732 (-) 441 WP_005725342.1 hypothetical protein -
  I927_RS07900 (I927_07890) - 1710744..1711319 (-) 576 WP_005725340.1 hypothetical protein -
  I927_RS07905 (I927_07895) - 1711316..1711651 (-) 336 WP_005725338.1 Mor transcription activator family protein -
  I927_RS07910 (I927_07900) - 1712046..1713776 (-) 1731 WP_230624005.1 DUF5906 domain-containing protein -
  I927_RS07915 (I927_07905) - 1713829..1714017 (-) 189 WP_005725335.1 hypothetical protein -
  I927_RS07920 (I927_07910) - 1714017..1714310 (-) 294 WP_046333743.1 hypothetical protein -
  I927_RS07925 (I927_07915) - 1714303..1714869 (-) 567 WP_052722512.1 host cell division inhibitor Icd-like protein -
  I927_RS07930 (I927_07920) - 1714950..1715141 (-) 192 WP_046333744.1 helix-turn-helix domain-containing protein -
  I927_RS07935 (I927_07925) - 1715665..1716900 (-) 1236 WP_046333746.1 tyrosine-type recombinase/integrase -
  I927_RS10950 - 1717468..1718747 (-) 1280 Protein_1555 TRAP transporter large permease -
  I927_RS07950 (I927_07940) - 1718744..1719304 (-) 561 WP_046333747.1 TRAP transporter small permease -
  I927_RS07955 (I927_07945) - 1719328..1720170 (-) 843 Protein_1557 TRAP transporter substrate-binding protein -
  I927_RS07960 (I927_07950) - 1720239..1720910 (+) 672 WP_046333749.1 cyclase family protein -
  I927_RS07965 (I927_07955) tpiA 1720883..1721971 (+) 1089 WP_080940664.1 triose-phosphate isomerase -
  I927_RS07970 (I927_07960) tal 1721989..1722939 (+) 951 WP_046333750.1 transaldolase -
  I927_RS07975 (I927_07965) rpoD 1723277..1725145 (-) 1869 WP_046333751.1 RNA polymerase sigma factor RpoD -
  I927_RS07980 (I927_07970) dnaG 1725221..1726969 (-) 1749 WP_046333752.1 DNA primase -
  I927_RS07985 (I927_07975) rpsU 1727085..1727300 (-) 216 WP_005717672.1 30S ribosomal protein S21 -
  I927_RS07990 (I927_07980) tsaD 1727519..1728550 (+) 1032 WP_046333753.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  I927_RS07995 (I927_07985) - 1728571..1728807 (+) 237 WP_046333754.1 hypothetical protein -
  I927_RS08000 (I927_07990) - 1728809..1729387 (+) 579 WP_005717659.1 thymidine kinase -
  I927_RS08005 (I927_07995) gorA 1729475..1730830 (-) 1356 WP_005748588.1 glutathione-disulfide reductase -
  I927_RS08010 (I927_08000) - 1730927..1731772 (-) 846 WP_025248561.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
  I927_RS08015 (I927_08005) pdxT 1731933..1732514 (-) 582 WP_005717651.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  I927_RS08020 (I927_08010) pdxS 1732517..1733404 (-) 888 WP_046333755.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  I927_RS08025 (I927_08015) - 1733515..1734927 (+) 1413 WP_046333756.1 PLP-dependent aminotransferase family protein -
  I927_RS08030 (I927_08020) - 1734959..1737520 (-) 2562 WP_005757394.1 penicillin-binding protein 1A -
  I927_RS08035 (I927_08025) - 1737656..1738429 (+) 774 WP_046333757.1 pilus assembly protein PilM -
  I927_RS08040 (I927_08030) - 1738469..1738984 (+) 516 WP_046333758.1 competence protein ComB -
  I927_RS08045 (I927_08035) - 1738984..1739508 (+) 525 WP_046333759.1 hypothetical protein -
  I927_RS08050 (I927_08040) - 1739511..1739873 (+) 363 WP_005748555.1 pilus assembly protein PilP -
  I927_RS08055 (I927_08045) comE 1739883..1741217 (+) 1335 WP_046333760.1 type IV pilus secretin PilQ Machinery gene

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49312.76 Da        Isoelectric Point: 8.0163

>NTDB_id=112360 I927_RS08055 WP_046333760.1 1739883..1741217(+) (comE) [Pasteurella multocida OH1905]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLYFAKAAEVMKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVIAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=112360 I927_RS08055 WP_046333760.1 1739883..1741217(+) (comE) [Pasteurella multocida OH1905]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTACAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGTATTTTGCCAAAGCCGCAGAGGTGATGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGAAGTTTAGCCGCGAACGGTTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACAG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCATCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.037

97.297

0.604
  comE Haemophilus influenzae 86-028NP

61.343

97.297

0.597
  comE Glaesserella parasuis strain SC1401

48.73

97.523

0.475
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369

Multiple sequence alignment